Anti-Vesicular GABA Transporter (VGAT) Antibody

半岛bd体育手机客户端 说明

一般描述

Vesicular GABA Transporter (unc-47) homologue is a 525 amino acid long protein belonging to the family of neurotransmitter transporter (1). VGAT functions in the uptake of GABA and glycine into synaptic vesicles. Since the protein is bound to synaptic vesicles, VGAT antibodies are excellent markers of presynaptic GABAergic and glycinergic neurons.

特异性

Specific for Vesicular GABA Transporter (VGAT). Vesicular GABA Transporter (unc-47) homologue is a 525 amino acid long protein belonging to the family of neurotransmitter transporter. The immunogen peptide shows no significant homology with other known proteins.
Antibody cross-reactivity with various species is not known. The immunogen sequence is 100% conserved in mouse, human and monkey.

免疫原

A 17 amino acid peptide sequence near the carboxy terminal region of rat VGAT (McIntire, S., 1997).

应用

Immunohistochemistry/Immunofluorescence:
2-20 ?g/mL of a previous lot was used on paraformaldehyde fixed sections.

ELISA:
1:10,000 – 1:100,000 dilution of a previous lot was used on ELISA, using 50-100 ng of control peptide (Cat. No. AG379) per well.

Western blot:
1-10 ?g/mL (Chemiluminescence technique) The expected molecular weight is approximately 55-60 kDa.

Optimal working dilutions must be determined by the end user.
This Anti-Vesicular GABA Transporter (VGAT) Antibody is validated for use in ELISA, IF, IH, WB for the detection of Vesicular GABA Transporter (VGAT).

质量

Evaluated by Western Blot on Rat retina lysates.

Western Blot Analysis:
1:500 dilution of this antibody detected Vesicular GABA Transporter on 10μg of rat retina lysates.

目标描述

57 kDa

外形

ImmunoAffinity Purified
Purified rabbit polyclonal in buffer containing PBS with 0.1% BSA.

分析说明

Control
Brain tissue, rat retina lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	rat, monkey, mouse, human
manufacturer/tradename	Chemicon?
technique(s)	ELISA: suitable immunofluorescence: suitable immunohistochemistry: suitable western blot: suitable
NCBI登记号	NM_080552.2
UniProt登记号	Q9H598
运输	dry ice
Gene Information	human ... SLC32A1(140679)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable