Donkey Anti-Goat IgG Antibody, HRP conjugate, Species Adsorbed

半岛bd体育手机客户端 说明

一般描述

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defense against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

应用

Immunohistochemistry:
1:500-1:5,000 dilution can be used.

Optimal working dilutions must be determined by end user.
Donkey anti-Goat IgG Antibody, HRP conjugate, Species Adsorbed is an antibody against Donkey Goat IgG for use in ELISA, IH & WB.

质量

Routinely tested on Enzyme-linked Immunosorbent Assay.

ELISA and Western Blots:
1:5,000-1:100,000 dilution can be used.

外形

Purified donkey secondary antibody IgG in lyophilized buffer 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA.
Reconstitution:
Reconstitute with 500 μL of sterile distilled water.
Immunoaffinity Purified and Immunodepletion

储存及稳定性

Maintain lyophilized product at 2-8°C for up to 12 months.
After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
WARNING: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.46

半岛bd体育手机客户端 性质

质量水平	100
生物来源	donkey
偶联物	peroxidase conjugate
抗体形式	purified immunoglobulin
antibody product type	secondary antibodies
克隆	polyclonal
species reactivity	goat
manufacturer/tradename	Chemicon?
technique(s)	ELISA: suitable immunohistochemistry: suitable western blot: suitable
同位素/亚型	IgG
运输	wet ice

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 3