Anti-Dopamine D1A Receptor Antibody, CT, cytoplasmic

半岛bd体育手机客户端 说明

特异性

Dopamine Receptor D1A. An antibody made to the D1R peptide has been shown to detect 2 proteins of 50 and 75 kDa in solubilized hippocampal membranes by Western blot. The 75 kDa band was not visible in striatum (Huang et al. 1992). Approximately 100-400 μg of protein/lane should be loaded to visualize the 75 kDa band. By immunohistochemistry D1R has been localized in various regions of the rat brain (Huang et al. 1992).

May recognize porcine or bovine (100 % similarity) or human (78% similarity).

免疫原

Epitope: C-terminus, cytoplasmic
A 13 amino acid peptide sequence (amino acids 403-415) from rat D1A (Monsma et al. 1990; Zhou et al. 1990; Zhou et al. 1992; O′Dowd et al. 1990). This peptide is predicted to be cytoplasmic.

应用

Research Category
Neuroscience
Anti-Dopamine D1A Receptor Antibody, C-terminus, cytoplasmic is an antibody against Dopamine D1A Receptor for use in ELISA, IH & WB.
Research Sub Category
Neurotransmitters & Receptors
Western blot: 1-10 μg/mL (Chemiluminescence technique). An antibody made to this peptide has been shown to detect 2 proteins of 50 and 75 kDa in solubilized hippocampal membranes. The 75 kDa band was not visible in striatum (Huang et al. 1992; O′Connel et al. 1995). Approximately 100-400 μg of protein/lane should be loaded to visualize the 75 kDa band (Huang et al. 1992; O′Connel et al. 1995) that is present in relatively small amounts.

Immunohistochemistry: 2-10 μg/mL on paraformaldehyde fixed sections.

ELISA: 0.5-1.0 μg/mL (1 μg/mL D1A peptide (Cat. Number AG259)/well)

Optimal working dilutions must be determined by end user.

外形

Affinity purified immunoglobulin. Liquid in phosphate buffered saline, pH 7.4, containing 0.1% BSA.

储存及稳定性

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity purified immunoglobulin
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	rat, pig
manufacturer/tradename	Chemicon?
technique(s)	ELISA: suitable immunohistochemistry: suitable western blot: suitable
NCBI登记号	NM_000794.3
UniProt登记号	P21728
运输	dry ice
Gene Information	human ... DRD1(1812)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable