Monoclonal Anti-p115/TAP antibody produced in mouse

半岛bd体育手机客户端 说明

一般描述

Monoclonal Anti-p115/TAP (mouse IgG1 isotype) is derived from the 5D6 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice. p115, a Golgin family tether is also referred as TAP1 (transcytosis associated protein). It is homologous to USO1 vesicle transport factor (Uso1p). It has a coiled coil dimerization domain, an N-terminal globular domain and a C-terminal acidic domain.

特异性

Monoclonal Anti-p115/TAP recognizes rat p115/TAP (approx. 95-100 kDa).

免疫原

purified rat p115/TAP.

应用

Monoclonal Anti-p115/TAP antibody produced in mouse may be used in:

• enzyme-linked immunosorbent assay (ELISA)
• immunoblotting
• immunoprecipitation
• immunocytochemistry

生化/生理作用

p115/TAP1 (transcytosis associated protein) plays an important role in endoplasmic reticulum to Golgi traffic and in intra?Golgi traffic. It is known to play a key role in the transport of endoplasmic reticulum (ER)/Golgi. p115 is highly essential for the docking or tethering step in intra-Golgi vesicular transport and transcytosis. It serve as a catalyst during the fusion mediated by soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE).

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

储存及稳定性

For continuous use, store at 2-8 ℃ for up to one month.For prolonged storage, freeze in working aliquots at ?20 ℃. Repeated freezing and thawing is not recom-mended. Storage in frost-free freezers is also notrecommended. If slight turbidity occurs upon prolongedstorage, clarify the solution by centrifugation beforeuse. Working dilutions should be discarded if not usedwithin 12 hours.

免责声明

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses,in vitrodiagnostic uses,ex vivoorin vivotherapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号

MFCD03792426

半岛bd体育手机客户端 性质

质量水平	200
生物来源	mouse
偶联物	unconjugated
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	5D6, monoclonal
形式	buffered aqueous solution
species reactivity	rat
technique(s)	immunocytochemistry: suitable immunoprecipitation (IP): suitable indirect ELISA: suitable microarray: suitable western blot: 2-4 μg/mL using cytosolic and membrane extracts of rat liver
同位素/亚型	IgG1
UniProt登记号	P41542
运输	dry ice
储存温度	?20℃
Gene Information	rat ... Uso1(56042)