pT7-FLAG^TM-2 Expression Vector

半岛bd体育手机客户端 说明

一般描述

pT7-FLAG-2 is a 4.8 kb E . coli expression vector used for cloning and cytoplasmic expression of a properly inserted open reading frame as a C-terminal FLAG^?fusion protein containing the FLAG epitope (DYKDDDDK). The promoter region of the very strong phage T7 promoter drives transcription of ORFFLAG fusion constructs. This vector requires the use ofE. colicells containing a source of the T7 polymerase, such as BL21(DE3)T1R cells, Catalog Number B2935.

The C-terminal FLAG fusion protein may be detected using Monoclonal ANTI-FLAG?M2, Catalog Number
F3165, and purified using the ANTI-FLAG M2 Affinity Gel, Catalog Number A2220.

Vector Maps and Sequences

组分

• pT7-FLAG-2 Expression Vector, 10 μg (P6867) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
• pT7-FLAG-2-BAP Control Vector 1 μg ( P7117) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.

法律信息

FLAG is a registered trademark of sigma-aldrich Co. LLC
pT7-FLAG is a trademark of sigma-aldrich Co. LLC

基本信息

NACRES

NA.85

半岛bd体育手机客户端 性质

质量水平	200
等级	for molecular biology
运输	dry ice
储存温度	?20℃

安全信息

储存分类代码

12 - Non Combustible Liquids