半岛bd体育手机客户端 说明
生化/生理作用
Methyl transferases catalyze the addition of methyl groups to nitrogen, carbon, sulfur, and oxygen atoms of small molecules, lipids, proteins, and nucleic acids. Eight mammalian protein arginine methyltransferases (PRMT) have been identified. PRMT1 is the predominant member of the methyl transferases, which catalyzes the protein arginine N-methylation reactions. PRMT1 is implicated in various cellular processes including: transcription, RNA processing, and signal transduction.
单位定义
The specific activity is ≥ 0.1 nmol/mgP/min. measured by3H-AdoMet incorporation into histone for 30 minues at 30 ℃.
外形
Solution of 50 mM Tris, pH 7.6, 5 mM DTT, 0.2% IGEPAL?CA-630, 150 mM NaCl, and 30% glycerol (w/v).
分析说明
The N-methyltransferase activity is determined by detecting the level of radiolabel transfer from3H-AdoMet (Methyl donor) to histone (Cat. No. H4380), which is arginine rich (methyl acceptor).
法律信息
IGEPAL is a registered trademark of Solvay
基本信息
NACRES | NA.32 |
半岛bd体育手机客户端 性质
质量水平 | 200 |
重组 | expressed inE. coli |
测定 | ≥90% (SDS-PAGE) |
形式 | buffered aqueous solution |
UniProt登记号 | Q63009 |
运输 | dry ice |
储存温度 | ?20℃ |
Gene Information | rat ... Prmt1(60421) |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |