Anti-PINK1 (N-terminal region) antibody produced in rabbit

半岛bd体育手机客户端 说明

一般描述

PTEN induced putative kinase 1 (PINK1) is a serine/threonine mitochondrial kinase. The 581 amino acid protein has an amino terminal mitochondrial target sequence, a putative transmembrane domain, a kinase domain and an autophosphorylation-regulating carboxy terminal domain. The gene encoding it is localized on human chromosome 1p36.12.

应用

Anti-PINK1 (N-terminal region) antibody produced in rabbit has been used in western blotting.

生化/生理作用

PTEN induced putative kinase 1 (PINK1) has a role in removing damaged mitochondria from cells. Mitochondrial damage triggers the accumulation of the protein in the outer mitochondrial membrane, wherein it undergoes autophosphorylation and dimerizes into a supermolecular protein complex. PINK1 then phosphorylates ubiquitin and tags damaged mitochondria for mitophagy. It negatively modulates glioblastoma growth. Mutations in the PINK1 gene have been associated with recessive, early-onset Parkinson′s disease. The protein is expressed in cancerous cells and has a role in cell survival.
PINK1 (PTEN induced putative kinase 1) has been found to protect neurons from stress-induced mitochondrial dysfunction and apoptosis. Genetic studies inDrosophilaindicate that PINK1 acts upstream of Parkin in a common pathway that influences mitochondrial morphology. PINK1 elicits protection in mouse primary neurons from the dopaminergic neurotoxin 1-methyl-4-phenylpyridine (MPP⁺)/1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) bothin vitroandin vivo. In response to enhanced proteasomal stressin vitro,PINK1 has been shown to be cleaved and localized to the mitochondria, and this correlates with increased expression of the processed PINK1 protein in Parkinson′s disease(PD) brain.

外形

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRES

NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	200
偶联物	unconjugated
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
形式	buffered aqueous solution
分子量	antigen ~60 kDa
species reactivity	human
包装	antibody small pack of 25 μL
增强验证	recombinant expression Learn more aboutAntibody Enhanced Validation
浓度	~1.5 mg/mL
technique(s)	western blot: 1-2 μg/mL using HEK-293T cell lysate expressing human PINK1
UniProt登记号	Q9BXM7
运输	dry ice
储存温度	?20℃
Gene Information	human ... PINK1(65018)

安全信息

储存分类代码	12 - Non Combustible Liquids
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)