半岛bd体育手机客户端 说明

一般描述

Anti-Phosphotyrosine antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the pT-154 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a tyrosine phosphorylated peptide. Members of protein-tyrosine kinases (PTKs) family consists of extra-cellular domain that is responsible for ligand binding, a trans-membrane domain, modular domain and an intracellular domain.

免疫原

tyrosine phosphorylated peptide.

应用

Anti-Phosphotyrosine antibody, Mouse monoclonal has been used in:

• enzyme linked immunosorbent assay (ELISA)
• western blot
• immunohistochemistry

生化/生理作用

Protein phosphorylation is a post-translational modification that regulates signal transduction. Phosphorylation of tyrosine residues in proteins has been associated with oncogenesis, cell growth and survival . Monoclonal Anti-Phosphotyrosine antibody is specific for rat and human proteins/peptides that contain phosphotyrosine residues. As determined by competitive ELISA, the product does not bind to non-phosphorylated tyrosine, phosphorylated serine or threonine, ATP or AMP.
Protein-tyrosine kinases (PTKs) are enzymes that catalyze the transfer of Γ-phosphate of ATP to tyrosine residues of protein substrates. The PTKs are responsible for many biological processes like cell cycle, proliferation, oncogenesis and development. They are tightly regulated by other kinases and by autophosphorylation activity. Monoclonal antibodies specific for phosphotyrosine are essential tools for the study of tyrosine phosphorylation in post-translational modification in many biological processes.

外形

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号	MFCD00164715
NACRES	NA.44

半岛bd体育手机客户端 性质

质量水平	200
偶联物	unconjugated
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	pT-154, monoclonal
浓度	~2 mg/mL
technique(s)	direct ELISA: suitable immunohistochemistry: suitable microarray: suitable western blot: 2-4 μg/mL using total extract of A431 cells stimulated by EGF
同位素/亚型	IgG2b
运输	dry ice
储存温度	?20℃

安全信息

储存分类代码	12 - Non Combustible Liquids
闪点(F)	Not applicable
闪点(C)	Not applicable