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Pyruvate Decarboxylase from baker's yeast (S. cerevisiae)

品牌
Sigma-Aldrich
CAS
9001-04-1
货号
P9474
规格纯度
ammonium sulfate suspension, 5.0-20.0 units/mg protein (biuret)
价格
1503.31 *本价格含增值税费
促销
服务
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数量
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半岛bd体育手机客户端 名称:
9001-04-1
Pyruvate Decarboxylase from baker's yeast ( S. cerevisiae)
2-Oxo-acid carboxy-lyase
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Pyruvate decarboxylase (PDC) usually appear in plant seeds at the time of germination, especially when the plant embryo is totally covered by an oxygen-impermeable testa.

应用

Pyruvate Decarboxylase from baker′s yeast (S. cerevisiae) has been used to evaluate the power of systematic identification of meaningful metabolic enzyme regulation (SIMMER) for finding unknown yeast regulatory interactions.
Pyruvate decarboxylase (PDC) is used to study residues involved in thiamine pyrophosphate (TPP) binding. It is used to study the regulation of fermentation pathways in plant species.

包装

500 units in glass bottle

生化/生理作用

Pyruvate decarboxylase (PDC) actively participates in the anaerobic metabolism of several bacteria, yeast and plant seeds.
Pyruvate decarboxylase (PDC) is a homotetrameric enzyme that catalyses the decarboxylation of pyruvic acid to acetaldehyde and carbon dioxide in the cytoplasm. Pyruvate decarboxylase depends on cofactors thiamine pyrophosphate (TPP) and magnesium. PDC contains a β-α-β structure, yielding parallel β-sheets.

单位定义

One unit will convert 1.0 μmole of pyruvate to acetaldehyde per min at pH 6.0 at 25 ℃.

外形

Suspension in 3.2 M (NH4)2SO4pH 6.5, stabilized with 5% glycerol, 5 mM potassium phosphate, 1 mM magnesium acetate, 0.5 mM EDTA, and 25 μM cocarboxylase.

制备说明

Isolated without the use of heavy metals.

基本信息

酶学委员会(EC)编号 4.1.1.1 ( BRENDA | IUBMB )
MDL编号 MFCD00132144
NACRES NA.54

半岛bd体育手机客户端 性质

质量水平 200
形式 ammonium sulfate suspension
specific activity 5.0-20.0 units/mg protein (biuret)
储存温度 2-8℃

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 2
闪点(F) Not applicable
闪点(C) Not applicable

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