16:0 DG

半岛bd体育手机客户端 说明

一般描述

In biochemical signaling, diacylglycerol (DAG) functions as a second messenger signaling lipid, and is a product of the hydrolysis of the phospholipid PIP2 (phosphatidylinositolbisphosphate) by the enzyme phospholipase C (PLC) (a membrane-bound enzyme) that, through the same reaction, produces inositol trisphosphate (IP3). Although inositol trisphosphate (IP3) diffuses into the cytosol, DAG remains within the plasma membrane due to its hydrophobic properties. IP3 stimulates the release of calcium ions from the smooth endoplasmic reticulum, whereas DAG is a physiological activator of protein kinase C (PKC). The production of DAG in the membrane facilitates translocation of PKC from the cytosol to the plasma membrane.
Diacylglycerol mimicks the effects of the tumor-promoting compounds phorbol esters.
16:0 DG (1,2-dipalmitoyl-sn-glycerol) is a longer acyl chains containing diacylglycerol.

应用

16:0 DG may be used :

• to spike brain samples for mass spectrometric analysis
• as a diacyl-glycerol source in diacylglycerol O-acyltransferase 1 (DGAT1) enzyme assay in human leukemic K562 cells
• in Golgi-like liposomes reconstitution

生化/生理作用

16:0 DG (1,2-dipalmitoyl-sn-glycerol) fails to prevent the late fission events in Golgi membrane. Supplementation of 16:0 DG promotes bacterial growth by preventing sporangia.

包装

5 mL Amber Glass Screw Cap Vial (800816P-200mg)
5 mL Amber Glass Screw Cap Vial (800816P-25mg)
5 mL Amber Glass Screw Cap Vial (800816P-10mg)

储存及稳定性

Diacylglycerols are conveniently stored in chloroform solutions in glass vials with PTFE-lined caps at -20℃. Under these conditions acyl migration is minimal. Avoid plastic when handling chloroform solutions.

其他说明

Effective concentration:
Most biological responses saturate at 20 to 250 μMsn-1,2-dioctanoylglycerol. Onlysn-1,2 isomers appear to be active.
Delivery to cells:
Dry samples of diacylglycerol in chloroform, using a stream of nitrogen. Dissolve the residue in an appropriate volume of ethanol or DMSO, then dilute to the desired aqueous medium.
Precaution:Since short chain Diacylglycerols mimic effects of the tumor-promoting phorbol diesters in a number of biological systems, extra care should be employed in their handling. Treatment of solutions, vessels and other articles with 1N NaOH before washing or discarding will destroy diacylglycerols.

基本信息

经验(实验)分子式	C35H68O5
分子量	568.91
NACRES	NA.25

半岛bd体育手机客户端 性质

测定	>99% (TLC)
形式	powder
包装	pkg of 1 × 10 mg (800816P-10mg) pkg of 1 × 200 mg (800816P-200mg) pkg of 1 × 25 mg (800816P-25mg)
manufacturer/tradename	Avanti Polar Lipids 800816P
运输	dry ice
储存温度	?20℃
InChI	1S/C35H68O5/c1-3-5-7-9-11-13-15-17-19-21-23-25-27-29-34(37)39-32-33(31-36)40-35(38)30-28-26-24-22-20-18-16-14-12-10-8-6-4-2/h33,36H,3-32H2,1-2H3
InChI key	JEJLGIQLPYYGEE-UHFFFAOYSA-N

安全信息

储存分类代码	13 - Non Combustible Solids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable