半岛bd体育手机客户端 说明
一般描述
O-GlcNAc transferase (OGT) catalyzes the addition of anN-acetylglucosamine residue to the amino acids serine or threonine. The enzyme is an homotrimer consisting of three subunits of 110 kDa each, with multiple tetratricopeptide (TPR) repeats.
免疫原
synthetic peptide corresponding to amino acids 1024-1037 of humanO-GlcNAc transferase, conjugated to KLH via an N-terminal added cysteine residue.
应用
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
生化/生理作用
O-linked β-N-acetyl glycosamine (O-GlcNAc) is involved in repressing transcription.O-GlcNAc transferase (OGT) interacts with a histone deacetylase complex by binding to the co repressor Sin3A. This interaction leads to the repression of transcription, after transcription factors and RNA polymerase II are modified by addition ofO-GlcNAc.O-GlcNAc modification reversibly inhibits proteosomal function in an ubiquitin-independent fashion.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD07370422 |
| NACRES | NA.41 |
半岛bd体育手机客户端 性质
| 生物来源 | rabbit |
| 质量水平 | 200 |
| 偶联物 | unconjugated |
| 抗体形式 | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen 110 kDa |
| species reactivity | mouse, human, rat |
| technique(s) | indirect immunofluorescence: 1:50-1:100 using A549 human lung carcinoma cells fixed with paraformaldehyde/triton western blot: 1:1,000-1:2,000 using HeLa cell nuclear extracts |
| UniProt登记号 | O15294 |
| 运输 | dry ice |
| 储存温度 | ?20℃ |
| Gene Information | human ... OGT(8473) mouse ... Ogt(108155) rat ... Ogt(26295) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
Sigma-Aldrich
m.cnreagent.com
