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位置: 首页> 品牌> Sigma-Aldrich> Anti-ABCA4 Antibody, clone 3F4

Anti-ABCA4 Antibody, clone 3F4

品牌
Sigma-Aldrich
货号
MABN2439M
规格纯度
clone 3F4, from mouse
价格
5854.74 *本价格含增值税费
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数量
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半岛bd体育手机客户端 名称:
Anti-ABCA4 Antibody, clone 3F4
ABC transporter A4, RIM ABC Transporter, RIM protein
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Retinal-specific ATP-binding cassette transporter (UniProt F1MWM0/P78363; also known as ATP-binding cassette sub-family A member 4, ABC transporter A4, RIM ABC transporter, RIM Protein) is encoded by the ABCA4 gene in bovine species. ABC transporter A4, a ~250 kDa single-chain, is a multi-pass membrane protein of the superfamily of ATP-binding cassette (ABC) transporters that is localized to the disk margins of vertebrate photoreceptor outer segments. It is implicated in the clearance of all-trans retinal derivatives from rod and cone photoreceptor cells following photoexcitation as part of the visual cycle. ABC transporter A4 is composed of two symmetrically organized halves, each comprising six membrane-spanning helices, a large glycosylated exocytoplasmic domain located inside the disk, and a cytoplasmic domain with an ATP-binding cassette. Mutations in ABCA4 are known to cause Stargardt macular degeneration, which is an autosomal recessive form of juvenile macular degeneration associated with a severe loss in vision. (Ref.: Tsybovsky, Y et al. (2010). Adv. Exp. Med. Biol. 703, 105-125).

特异性

Clone 3F4 detects ABC transporter (ABCA4) in Bovine and Human cells. It targets an epitope within 9 amino acids from the C-terminal end.

免疫原

Whole purified bovine disk rim protein.

Epitope: domain of:

应用

Immunofluorescence Analysis: A 1:50 dilution from a representative lot detected ABCA4 in mouse retina cells.

Immunofluorescence Analysis: A 1:250 dilution from a representative lot detected ABCA4 in mouse retina cells (Courtesy of Dr. Robert Molday, Ph.D., University of British Columbia, Canada).

Immunoprecipitation Analysis: A representative lot detected ABCA4 in Immunoprecipitation applications (Bungert, S., et. al. (2001). J Biol Chem. 276(26):23539-46).

Western Blotting Analysis: A representative lot detected ABCA4 in Western Blotting applications (Molday, L.L., et. al. (2000). Nat Genet. 25(3):257-8; Illing, M., et. al. (1997). J Biol Chem. 272(15):10303-10; Beharry, S., et. al. (2004). J Biol Chem. 279(52):53972-9).

Immunohistochemistry Analysis: A representative lot detected ABCA4 in Immunohistochemistry applications (Molday, L.L., et. al. (2000). Nat Genet. 25(3):257-8).
Anti-ABCA4, clone 3F4, Cat. No. MABN2439, is a highly specific mouse monoclonal antibody that targets ABCA4 (RIM) and has been tested in Immunohistochemistry, Immunoprecipitation, Immunofluorescence, and Western Blotting.
Research Category
Neuroscience

质量

Evaluated by Western Blotting in human retina tissue lysate.

Western Blotting Analysis: 1 ?g/mL of this antibody detected ABCA4 in 10 ?g of human retinal tissue lysate.

目标描述

~220 kDa observed; 257.38 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702

半岛bd体育手机客户端 性质

质量水平 100
生物来源 mouse
抗体形式 purified immunoglobulin
antibody product type primary antibodies
克隆 3F4, monoclonal
species reactivity mouse, bovine, human
包装 antibody small pack of 25 μg
technique(s) immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型 IgG1κ
UniProt登记号 F1MWM0

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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