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Anti-IST1 Homolog

品牌
Sigma-Aldrich
货号
ABS1633M
规格纯度
serum, from rabbit
价格
5016.79 *本价格含增值税费
促销
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数量
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半岛bd体育手机客户端 名称:
Anti-IST1 Homolog
hIST1, Putative MAPK-activating protein PM28
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

IST1 homolog (UniProt: P53990; also known as hIST1, Putative MAPK-activating protein PM28) is encoded by the IST1 (also known as KIAA0174) gene (Gene ID: 9798) in human. IST1 is an ESCRT-III-like protein that is involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis. IST1 is also involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. During late anaphase, it is involved in nuclear envelope reassembly and mitotic spindle disassembly together with the ESCRT-III complex: IST1 acts by mediating the recruitment of SPAST to the nuclear membrane, leading to microtubule severing. It is shown to regulate early endosomal tubulation together with the ESCRT-III complex by mediating the recruitment of SPAST. Six isoforms of IST1 homolog have been described that are generated by alternative splicing.

特异性

This rabbit polyclonal antibody detects IST1 homolog in human cells. It targets an epitope within the first 189 amino acids from the N-terminal half.

免疫原

A recombinant fragment corresponding to the first 189 amino acids from the N-terminal half of human IST1 homolog.

应用

Anti-IST1 Homolog, Cat. No. ABS1633,is a rabbit polyclonal antibody that detects IST1 homolog and has been tested for use in Immunofluorescence and Western Blotting.
Western Blotting Analysis: A 1:5,000 dilution from a representative lot detected IST1 Homolog in 10 ?g of HeLa cell lysate.

Western Blotting Analysis: A representative lot detected IST1 Homolog in Western Blotting applications (Bajorek, M., et. al. (2009). Nat Struct Mol Biol. 16(7):754-62; Bajorek, M., et. al. (2009). Mol Biol Cell. 20(5):1360-73).

Immunofluorescence Analysis: A representative lot detected IST1 Homolog in Immunofluorescence applications (Bajorek, M., et. al. (2009). Mol Biol Cell. 20(5):1360-73).

质量

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected IST1 Homolog in 10 ?g of Raji cell lysate.

目标描述

~50 kDa observed; 39.75 kDa calculated.. Uncharacterized bands may be observed in some lysate(s).

外形

Affinity Purified

储存及稳定性

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702

半岛bd体育手机客户端 性质

生物来源 rabbit
质量水平 100
抗体形式 serum
antibody product type primary antibodies
克隆 polyclonal
species reactivity human
species reactivity (predicted by homology) chimpanzee (based on 100% sequence homology)
technique(s) immunofluorescence: suitable
western blot: suitable
同位素/亚型 IgG
NCBI登记号 NP_001257904
UniProt登记号 P53990
运输 ambient

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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