半岛bd体育手机客户端 说明
一般描述
Etanercept is a chimeric fusion protein consisting of the extracellular ligand-binding portion of the p75 TNF receptor linked to the Fc portion of human IgG1. This Fc component contains the hinge, CH2 and CH3 regions, but not the CH1 region of IgG1. Etanercept binds to TNF-alpha and blocks its interactions with receptors. Hence, it is considered a TNF inhibitor that functions as a decoy receptor that binds to TNF. Although it mimics the inhibitory effects of naturally occurring soluble TNF receptors, the difference is that Etanercept has a greatly extended half-life in the bloodstream. Its speci fic activity is reported to be about 1.7 x 106 units/mg. Etanercept is shown to modulate biological responses produced by TNF, including expression of adhesion molecules responsible for leukocyte migration, serum levels of cytokines, and serum levels of matrix metalloproteinase-3. It is also shown to affect animal models of inflammation, including murine collagen-induced arthritis. Clone ETA63C8 can bind to Etanercept and block its activity.
特异性
Clone ETA63C8 detects Etanercept in human samples. It can bind to Etanercept and block its activity.
免疫原
Chimeric fusion protein cotaining the extracellular ligand-binding portion of the p75 TNF receptor linked to the Fc portion of human IgG1.
应用
Neutralizing Analysis: A representative lot neutralized Etanercept in Neutralizing applications (Detrez, I., et. al. (2016). J Immunol. 196(6):2879-84).
ELISA Analysis: A representative lot detected Etanercept in ELISA applications (Detrez, I., et. al. (2016). J Immunol. 196(6):2879-84).
ELISA Analysis: 4 ?g/mL from a representative lot detected Etanercept in human serum with AS (ankylosing spondylitis) (Courtesy of Professor Ann Gils, Ph.D., KU Leuven Belgium).
Anti-Etanercept, clone ETA63C8, Cat. No. MABF1973, is a mouse monoclonal antibody that can bind to Etanercept and has been tested for use in ELISA and Neutralizing.
Research Category
Inflammation & Immunology
质量
Isotype testing: Identity Confirmation by Isotyping Test.
Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1.
目标描述
150 kDa calculated.
外形
Protein G purified
Purified mouse monoclonal antibody IgG1 in PBS without azide.
Format: Purified
储存及稳定性
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
其他说明
Concentration: Please refer to lot specific datasheet.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
半岛bd体育手机客户端 性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | ETA63C8, monoclonal |
| species reactivity | human |
| 包装 | antibody small pack of 25 μL |
| technique(s) | ELISA: suitable neutralization: suitable |
| 同位素/亚型 | IgG1κ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
