您好,欢迎来到半岛电竞官方网址 ! [ 登录] [ 免费注册]
半岛电竞官方网址
位置: 首页> 品牌> Sigma-Aldrich> Anti-CCAR1 Antibody, clone 4B9.1

Anti-CCAR1 Antibody, clone 4B9.1

品牌
Sigma-Aldrich
货号
MABE1671M
规格纯度
clone 4B9.1, from mouse
价格
1415.25 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
- +
半岛bd体育手机客户端 名称:
Anti-CCAR1 Antibody, clone 4B9.1
Cell division cycle and apoptosis regulator protein 1, Cell cycle and apoptosis regulatory protein 1, CARP-1, Death inducer with SAP domain
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Cell division cycle and apoptosis regulator protein 1 (UniProt: Q8IX12; also known as Cell cycle and apoptosis regulatory protein 1, CARP-1, Death inducer with SAP domain, CCAR1) is encoded by the CCAR1 (also known as CARP1, DIS) gene (Gene ID: 55749) in human. CCAR1 is a cytoplasmic protein that localized to the perinuclear region. It is expressed in various epithelial cancer cell lines, including breast, colon, prostate, pancreatic and leukemia. Its expression is shown to be regulated by growth factors. Two isoforms of CCAR1 have been reported that are generated by alternative splicing. CCAR1 associates with components of the Mediator and p160 coactivator complexes that play a role as intermediaries transducing regulatory signals from upstream transcriptional activator proteins to basal transcription machinery at the core promoter. CCAR1 is recruited to endogenous nuclear receptor target genes in response to the appropriate hormone. It also functions as a p53 coactivator and may play a role in transcriptional regulation. It is reported to be involved in apoptosis signaling in the presence of the reinoid CD437. In association with CALCOCO1, it enhances GATA1- and MED1-mediated transcriptional activation from the gamma-globin promoter during erythroid differentiation of K562 erythroleukemia cells. CCAR1 can act as a coactivator and corepressor of AR-mediated transcription and contribute to chromatin looping and AR transcription complex assembly by stabilizing AR-GATA2 association on chromatin and facilitating MED1 and RNA polymerase II recruitment to AR-binding sites.

特异性

Clone 4B9.1 detects Cell division cycle and apoptosis regulator protein 1 (CCAR1) in human cells. It targets an epitope witihin 173 amino acids from the N-terminal half.

免疫原

GST/His-tagged recombinant fragment corresponding to 173 amino acids from the N-terminal half of human Cell division cycle and apoptosis regulator protein 1 (CCAR1).

应用

Anti-CCAR1, clone 4B9.1, Cat. No. MABE1671, is a highly specific mouse monoclonal antibody that targets Cell division cycle and apoptosis regulator protein 1 and has been tested in Immunocytochemistry and Western Blotting.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected CCAR1 in HeLa, A431, and HUVEC cells.

Western Blotting Analysis: 0.5 ?g/mL from a representative lot detected CCAR1 in 10 ?g of HepG2 cell lysate.

Research Category
Epigenetics & Nuclear Function

质量

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 ?g/mL of this antibody detected CCAR1 in 10 ?g of HeLa cell lysate.

目标描述

~135 kDa observed; 132.82 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Protein G purified
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702

半岛bd体育手机客户端 性质

质量水平 100
生物来源 mouse
抗体形式 purified immunoglobulin
antibody product type primary antibodies
克隆 4B9.1, monoclonal
species reactivity human
包装 antibody small pack of 25 μg
technique(s) immunohistochemistry: suitable
western blot: suitable
同位素/亚型 IgG2bκ
NCBI登记号 NP_060707
UniProt登记号 Q8IX12

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

Sigma-Aldrich

推荐半岛bd体育手机客户端
Baidu
map