ChemiSCREEN Membrane Preparation Recombinant Human MC₁Melanocortin Receptor

半岛bd体育手机客户端 说明

一般描述

The melanocortin-1 receptor (MC₁) is a member of the G-protein-coupled receptor family of proteins that is responsible for regulating skin and hair color. The MC₁receptor is regulated by the physiological agonists alpha-melanocyte-stimulating hormone (alphaMSH) and adrenocorticotropic hormone (ACTH), and antagonist agouti signaling protein (ASP) (Rouzud et al., 2005). In 1995 a landmark study demonstrated that over 80% of humans with red hair or fair skin have a dysfunctional variant of the MC₁gene (Valverde et al., 1995). MC₁is, therefore, a major determinant of sun sensitivity and genetic risk factor for melanoma and non-melanoma skin cancer. Millipore′s MC₁membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of agonists and antagonists of MC₁. The membrane preparations exhibit a Kd of 1.3nM for [¹²⁵I]-[Nle⁴, D-Phe⁷]-α-MSH (NDP-αMSH). With 0.3 nM [¹²⁵I]-NDP-αMSH, 5μg/well MC₁Membrane Prep typically yields greater than 50-fold signal-to-background ratio.

应用

Radioligand binding assay and GTPγS binding.
Human MC1 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.

生化/生理作用

• GPCR Class: A
• Protein Target: MC1
• Target Sub-Family: Melanocortin

外形

Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives. Packaging method: Membranes protein were adjusted to 1 mg/mL in packaging buffer, and dispensed at 1 mL/vial. Vials were rapidly frozen, and stored at -80℃.

储存及稳定性

Maintain frozen at -70℃ for up to 2 years. Do not freeze and thaw.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

基本信息

eCl@ss

32161000

半岛bd体育手机客户端 性质

质量水平	100
生物来源	human
重组	expressed in Chem-1 cells
manufacturer/tradename	ChemiScreen Chemicon?
technique(s)	ligand binding assay: suitable (GTPγS) radioligand binding assay (RLBA): suitable
NCBI登记号	NM_002386.2
UniProt登记号	Q01726

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable