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位置: 首页> 品牌> Sigma-Aldrich> ChemiSCREEN Human H2Histamine Receptor Membrane Preparation

ChemiSCREEN Human H2Histamine Receptor Membrane Preparation

品牌
Sigma-Aldrich
货号
HTS086M
规格纯度
Human H2 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
价格
10463.8 *本价格含增值税费
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半岛bd体育手机客户端 名称:
ChemiSCREEN Human H 2Histamine Receptor Membrane Preparation
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Full-length human HRH2 cDNA encoding H2
The histamine H2 receptor is a widely distributed G-protein coupled receptor, which is important in regulating a host of physiologic actions extending from gastric acid secretion to gastrointestinal motility (Del Valle and Gantz, 1997). Histamine mediated activation of its receptor leads to signaling through both adenylate cyclase and phosphoinositide /protein kinase C second messenger systems (Hill, 1990). Antagonists for this receptor have proven to be effective therapy for acid peptic disorders of the GI tract. Certain antagonists are used in the treatment of neuropsychiatric and neurological diseases e.g. schizophrenia, Alzheimer′s disease and Parkinson′s disease. Chemicon′s H2 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of agonists and antagonists at H2. The membrane preparations exhibit a Kd of 0.8 nM for [125I]-Iodoaminopotentidine. With 0.65 nM [125I]-Iodoaminopotentidine, 10 ?g/well H2 Membrane Prep typically yields greater than 7-fold signal-to-background ratio.

应用

Human H2 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.

生化/生理作用

Target Sub-Family: Histamine
Protein Target: H2
GPCR Class: A

质量

Table 1. Signal:background and specific binding values obtained in a competition binding assay with H2 membrane prep and unlabeled tiotidine.

10 ?g/well
Signal:Background 11.68
Specific Binding (cpm) 4152


SPECIFICATIONS: 1 unit = 10 ?g
Bmax for [125I] Iodoaminopotentidine binding: 0.46 pmol/mg protein
Kd for [125I] Iodoaminopotentidine binding: ~0.8 nM

半岛bd体育手机客户端 规格

Inucbation Conditions
Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, an FC 96-well harvest plate (Millipore cat. # MAHF C1H) is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.

Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C
Radioligand: [125I] Iodoaminopotentidine (Amersham#: IM264)
Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl , 0.1% BSA, filtered and stored at 4°C.

外形

One package contains enough membranes for at least 200 assays (units), where a unit is the amount of membrane that will yield greater than 7-fold signal:background with 125I-labeled Iodoaminopotentidine at 0.65 nM

Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
Packaging method: Membranes protein were adjusted to 1 mg/mL in packaging buffer, and dispensed at 1 mL/vial. Vials were rapidly frozen, and stored at -80oC.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32161000

半岛bd体育手机客户端 性质

质量水平 100
生物来源 human
重组 expressed in Chem-2 cells
manufacturer/tradename ChemiScreen
Chemicon?
technique(s) ligand binding assay: suitable (GTPγS)
radioligand binding assay (RLBA): suitable
NCBI登记号 NM_022304.1
UniProt登记号 P25021
运输 dry ice

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 2
闪点(F) Not applicable
闪点(C) Not applicable

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