Methionine Aminopeptidase fromPyrococcus furiosus

半岛bd体育手机客户端 说明

一般描述

Methionine aminopeptidase fromPyrococcus furiosusis a 32 kDa thermostable enzyme. It belongs to type 2a class of methionine aminopeptidase. Methionine aminopeptidase maintains protein homeostasis and coordinates posttranslational modification of proteins in eukaryotes.
X-ray crystallography of the structure of methionine aminopeptidase fromPyrococcus furiosusor PfMAP was performed at a resolution of 1.75A and showed that the protein consists of a catalytic domain containing two cobalt ions in the active site and a unique insertion domain which is specific to the prokaryotic form of the protein.

应用

Methionine Aminopeptidase fromPyrococcus furiosushas been used in a study to analyze the binding of Co(II)-specific inhibitors to the methionyl aminopeptidases fromEscherichia coliandPyrococcus furiosus. It has also been used in a study to examine the binding of a new class of pseudopeptide analog inhibitors.

生化/生理作用

Thermostable methionine aminopeptidase, which specifically liberates the N-terminal methioinine from proteins and peptides.

单位定义

One unit will hydrolyze 1 μmol of Met from Met-Pro-Ala-Ala-Gly in 1 minute at pH 7.5 at 37 ℃.

外形

Solution containing 0.01% Tween^?20, 0.1 mM CoCl₂, and 10 mM Tris-HCl, pH 7.5.

法律信息

TWEEN is a registered trademark of Croda International PLC

基本信息

酶学委员会(EC)编号	3.4.11.18 ( BRENDA | IUBMB )
MDL编号	MFCD03457470
NACRES	NA.54

半岛bd体育手机客户端 性质

质量水平	200
重组	expressed inE. coli
测定	≥93% (SDS-PAGE)
形式	solution
specific activity	0.5 units/mg protein
分子量	37 kDa by SDS-PAGE
UniProt登记号	P56218
异质活性	Other proteases, none detected
运输	dry ice
储存温度	?20℃
Gene Information	Pyrococcus furiosusDSM 3638 ... PF0541(1468383)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable