Monocyte Chemotactic Protein-1 human

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一般描述

Monocyte chemoattractant protein-1 (MCP-1) or monocyte chemotactic and activating factor (MCAF) is part of the C-C chemokine family. The gene encoding this protein is localized on human chromosome 17q12.

应用

Monocyte chemotactic protein-1 (MCP-1) has been used to study its effects on the motility of lymphoblastoid cell line NC–NC cells.

生化/生理作用

The biological activity is measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR2A. The ED₅₀ for this effect is typically 5-30 ng/mL.

Measured by its ability to chemoattract 2-day cultured human monocytes, Matsushima, K., et al. (1989) J. Exp. Med. 169:1485, the ED₅₀ for this effect is typically 5-20 ng/mL.

Monocyte chemoattractant protein-1 (MCP-1) triggers the activation of monocytes by triggering degranulation as well as respiratory burst activity in monocytes. It facilitates monocytes in inhibiting or enhancing the growth of specific brain tumor cell lines. MCP-1 chemoattracts monocytes, macrophages, T cells and natural killer cells. The protein is overexpressed in atherosclerosis, idiopathic pulmonary fibrosis, rheumatoid arthritis and delayed hypersensitivity reactions. It is also implicated in Kawasaki disease.

外形

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin.

基本信息

MDL编号

MFCD00131621

半岛bd体育手机客户端 性质

质量水平	200
生物来源	human
重组	expressed inE. coli
测定	≥97% (SDS-PAGE and N-terminal analysis)
形式	lyophilized powder
效能	5-60 ng/mL EC50
分子量	8.7 kDa
包装	pkg of 10 μg
technique(s)	cell culture | mammalian: suitable
杂质	endotoxin, tested
UniProt登记号	P13500
储存温度	?20℃
Gene Information	human ... CCL2(6347)

安全信息

储存分类代码	13 - Non Combustible Solids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, type N95 (US)