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Matrix Metalloproteinase-1 human

品牌
Sigma-Aldrich
货号
M9195
规格纯度
recombinant, expressed in NSO cells, >95% (SDS-PAGE), buffered aqueous solution
价格
9501.04 *本价格含增值税费
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数量
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半岛bd体育手机客户端 名称:
Matrix Metalloproteinase-1 human
MMP-1
Interstitial Collagenase
Collagenase-1
Matrix Metalloproteinase-1 人
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

生化/生理作用

MMP-1 initiates the breakdown of interstitial collagens.

外形

Supplied as a 0.2 μm filtered solution of 25 mM MES, 10 mM calcium chloride, 150 mM sodium chloride, 0.05% Brij?L23, pH 5.5.

分析说明

The biological activity is measured by its ability to cleave a fluorogenic peptide substrate.

其他说明

Activity Assay Protocol

Materials:

  • Assay Buffer: 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% Brij-35, pH 7.5 (TCNB)
  • Recombinant Human MMP-1 (rhMMP-1) (Cat. No. M9195)
  • p-aminophenylmercuric acetate (APMA), (Cat. No. A9563), 100 mM stock in DMSO
  • Substrate: MCA-Lys-Pro-Leu-Gly-Leu-DPA-Ala-Arg-NH2
  • F16 Black Maxisorp Plate
  • Fluorescent Plate Reader or equivalent

Assay:
1. Dilute rhMMP-1 to 50 μg/mL in Assay Buffer.
2. Activate 50 μg/mL rhMMP-1 by adding APMA to a final concentration of 1 mM.
3. Incubate at 37 ℃ for 2 hours.
4. Dilute activated rhMMP-1 to 1 ng/μL in Assay Buffer.
5. Dilute Substrate to 20 μM in Assay Buffer.
6. Load into a black well plate 50 μL of 1 ng/μL rhMMP-1 and start the reaction by adding 50 μL of 20 μM Substrate. Include a Substrate Blank containing 50 μL Assay Buffer, 50 μL Substrate, and no rhMMP-1.
7. Read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes.
8. Calculate specific activity:
Specific Activity (pmol/min/μg) =Adjusted V max* (RFU/min) x Conversion Factor** (pmol/RFU) / amount of enzyme (μg)

*Adjusted for Substrate Blank
**Derived using calibration standard MCA-Pro-Leu-OH.

Final Assay Conditions:

Per Well:
rhMMP-1: 0.050 μg
Substrate: 10 μM

法律信息

Brij is a registered trademark of Croda International PLC

基本信息

酶学委员会(EC)编号 3.4.24.7 ( BRENDA | IUBMB )
MDL编号 MFCD00130830
NACRES NA.32

半岛bd体育手机客户端 性质

质量水平 200
重组 expressed in NSO cells
测定 >95% (SDS-PAGE)
形式 buffered aqueous solution
enzyme activity ≥400 pmol/min-μg
分子量 apparent mol wt 52-55 kDa
UniProt登记号 P03956
运输 dry ice
储存温度 ?70℃
Gene Information human ... MMP1(4312)

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 2
闪点(F) Not applicable
闪点(C) Not applicable
个人防护装备 Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Sigma-Aldrich

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