Macrophage Colony-Stimulating Factor from mouse

半岛bd体育手机客户端 说明

应用

Macrophage Colony-Stimulating Factor from mouse has been used:

• in the initiation of osteoclastogenesis in bone marrow monocyte cells
• to stimulate osteoclast differentiation in non-adherent hematopoietic cell
• for the generation of blood-derived stem cells

生化/生理作用

Macrophage Colony Stimulating Factor (M-CSF), also known as CSF-1, can be produced by a number of cells, including fibroblasts, monocytes, activated macrophages, secretory epithelial cells of the endometrium, endothelial cells activated by LPS or cytokines, and bone marrow stromal cells. In addition to its namesake activity, M-CSF can stimulate the proliferation of isolated macrophages, augment the production and release of cytokines and other inflammatory modulators from macrophages, enhance macrophage antibody-dependent cell-mediated cytotoxicity, prime and enhance macrophages in their ability to kill tumor cells and other microorganisms, stimulate pinocytosis, and support osteoclast differentiation. M-CSF binds and activates a 165 kDa glycoprotein of the receptor tyrosine kinase subclass III or the RTK subfamily. Due to alternative splicing of a single M-CSF gene and due to variations in glycosylation, the molecular weight of natural soluble M-CSF ranges from 44-86 kDa. Human and mouse M-CSF share approximately 80% sequence homology in the conserved N-terminal region of 150 amino acids. Although human M-CSF is active in murine systems, mouse M-CSF appears to be species-specific in its actions.

外形

Lyophilized from a sterile filtered buffered aqueous solution.

分析说明

The proliferative activity is tested by the dose-dependent stimulation of the murine monocytic cell line, M-NFS-60.

基本信息

EC 号	214-684-5
MDL编号	MFCD00166591
eCl@ss	32190102

半岛bd体育手机客户端 性质

质量水平	200
生物来源	mouse
重组	expressed inE. coli
测定	≥98% (SDS-PAGE)
形式	lyophilized powder
效能	0.500-3.000 ng/mL ED50
质量	endotoxin tested
分子量	dimer 18.2 kDa (containing 156 amino acid residues)
包装	pkg of 10 μg
technique(s)	cell culture | mammalian: suitable
杂质	≤1.000 EU/μg
颜色	white
UniProt登记号	P07141
储存温度	?20℃
Gene Information	mouse ... Csf1(12977)

安全信息

象形图
警示用语：	Warning
危险声明	H315 - H319 - H335
预防措施声明	P261 - P305 + P351 + P338
危险分类	Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
储存分类代码	13 - Non Combustible Solids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	dust mask type N95 (US), Eyeshields, Gloves