GCK Protein, active, 10 μg

半岛bd体育手机客户端 说明

一般描述

N-terminal GST- tagged, recombinant, human GCK amino acids 1-473, expressed by baculovirus in Sf21 cells.
Product Source: Recombinant protein expressed in Sf21 cells

应用

Research Category
Metabolism
Research Sub Category
Cardiovascular Disease

Metabolic Disorders

生化/生理作用

Protein Target: GCK
Target Sub-Family: STE

质量

Routinely evaluated by phosphorylation of 0.8 mg/mL myelin basic protein.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3 μg of active GCK.

单位定义

Specific Activity: where one unit of GCK activity is defined as 1nmol phosphate incorporated into 0.8 mg/mL myelin basic protein per minute at 30°C with a final ATP concentration of 100 μM.

外形

Purified using glutathione-agarose. Purity 52% by SDS-PAGE and Coomassie blue staining.

储存及稳定性

Stable for 6 months at -70 °C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 °C.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160405
NACRES	NA.32

半岛bd体育手机客户端 性质

质量水平	100
生物来源	human
specific activity	2337 U/mg
分子量	Mw80.6 kDa
manufacturer/tradename	Upstate?
technique(s)	activity assay: suitable (kinase)
NCBI登记号	NM_004579.2
UniProt登记号	Q12851