半岛bd体育手机客户端 说明
一般描述
N-terminal 6His-tagged, recombinant, human TAO3 amino acids 1-411; N47S.
Product Source: Recombinant, human TAO3 amino acids 1-411; N47S. Expressed by baculovirus in Sf21 cells.
应用
Research Category
Metabolism
Research Sub Category
Cardiovascular Disease
Metabolic Disorders
生化/生理作用
Protein Target: TAO3
Target Sub-Family: STE
质量
Routinely evaluated by phosphorylation of 0.8 mg/mL myelin basic protein.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3μg of TAO3.
单位定义
Specific Activity: where one unit of TAO3 activity is defined as 1nmol phosphate incorporated into 0.8mg/ml myelin basic protein per minute at 30°C with a final ATP concentration of 100μM.
外形
10 μg of enzyme in 6.3 μL of 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij 35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Ni2+/NTA-agarose
储存及稳定性
Stable for 6 months at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160405 |
| NACRES | NA.32 |
半岛bd体育手机客户端 性质
| 质量水平 | 100 |
| 生物来源 | human |
| 测定 | 84% (Coomassie blue staining, SDS-PAGE) |
| specific activity | 915 U/mg |
| 分子量 | Mw50.2 kDa |
| manufacturer/tradename | Upstate? |
| technique(s) | activity assay: suitable (kinase) |
| NCBI登记号 | NM_016281.3 |
| UniProt登记号 | Q9H2K8 |
| Gene Information | human ... TAOK3(51347) |
Sigma-Aldrich
m.cnreagent.com
