半岛bd体育手机客户端 说明
一般描述
Recombinant,Streptococcus pneumoniaeβ1,4-Galactosidase expressed inE. coli. Catalyzes the hydrolysis of non-reducing terminal β1,4-linked galactose. This enzyme is not known to cleave substituted or branched galactose. Inhibited by fucose attached to penultimate sugar.
Note: 1 mU = 1 milliunit.
应用
Recommended reaction buffer: 50 mM sodium phosphate buffer, pH 6.0
警告
Toxicity: Standard Handling (A)
单位定义
One unit is defined as the amount of enzyme that will catalyze the release of 1.0 ?molp-nitrophenol fromp-nitrophenyl-β-D-galactopyranoside per min at 37°C, pH 5.0.
外形
In 25 mM NaCl, 20 mM Tris-HCl, pH 7.5. Sterile solution.
其他说明
Kierman, U.A., et al. 2004.Proteomincs4, 1825.
Prime, S., et al. 1996.J. Chromatogr. A.720,263.
Dwek, R.A., et al. 1993.Annu. Rev. Biochem.62,65.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
酶学委员会(EC)编号 | 3.2.1.23 ( BRENDA | IUBMB ) |
半岛bd体育手机客户端 性质
形式 | liquid |
质量水平 | 200 |
specific activity | ≥1.5 units/mL ≥6 units/mg protein |
manufacturer/tradename | Calbiochem? |
储存条件 | do not freeze |
异质活性 | N-acetylglucosaminidase, α-galactosidase, α-mannosidase, neuraminidases, proteases, none detected |
储存温度 | 2-8℃ |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich