半岛bd体育手机客户端 说明
一般描述
Anti-GABA, rabbit polyclonal, recognizes GABA in rat thalamus and cerebellum. It is validated for use in immunohistochemistry on frozen sections.
Rabbit polyclonal antibody supplied as lyophilized undiluted serum. Recognizes GABA.
Recognizes GABA in rat thalamus and cerebellum.
免疫原
Rat
GABA
包装
100 μL in Plastic ampoule
应用
Frozen Sections (1:12,000-1:15,000, ABC detection or 1:3000-1:5000, PAP detection; see comments)
警告
Toxicity: Standard Handling (A)
外形
Undiluted serum.
重悬
Reconstitute the lyophilized antibody with 100 ?l sterile distilled H?O. For storage, dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
分析说明
Positive Control
Rat thalamus or cerebellum
其他说明
Antibody specificity was examined in the rat thalamus and cerebellum. Glutaraldehyde is a necessary component of fixation for use with this antibody. Higher concentrations of glutaraldehyde (1%-2%) may be used as needed. The specificity of the antibody for GABA was evaluated using a competitive inhibition ELISA. A GABA conjugate control completely eliminated labeling, while 1000-fold excess of the following conjugates: alanine, aspartate, beta alanine, glutamate, glycine, taurine, and tyrosine did not inhibit the binding to the GABA conjugate control. Antibody should be titrated for optimal results in individual systems.
Sudhof, T.C. 1995.Nature375,645.
Hokfelt, T., et al. 1987.Experientia Suppl.56,154.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
半岛bd体育手机客户端 性质
| 生物来源 | rabbit |
| 质量水平 | 100 |
| 抗体形式 | serum |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 形式 | lyophilized |
| 不包含 | preservative |
| species reactivity | rat |
| manufacturer/tradename | Calbiochem? |
| 储存条件 | OK to freeze |
| 同位素/亚型 | IgG |
| 运输 | ambient |
| 储存温度 | ?20℃ |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
