Anti-EGFR (Ab-1) Mouse mAb (528)

半岛bd体育手机客户端 说明

一般描述

Anti-EGFR (Ab-1), mouse monoclonal, clone 528, recognizes EGF receptor protein in A431 cells and normal skin. It is validated for use in ELISA, IF, IP, IHC (frozen,paraffin) & neutralization studies.
Recognizes the EGF receptor protein in A431 cells and normal skin.
Purified mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~170 kDa EGFR protein.

免疫原

partially purified, human EGFR from human A431 cells

包装

100 μg in Plastic ampoule

应用

Flow Cytometry (5 ?g/ml)

Frozen Sections (2.5 ?g/ml)

Immunofluorescence (5 ?g/ml)

Immunoprecipitation (1 ?g/sample)

Neutralization Studies (see comments and application references)

Paraffin Sections (see application references)

警告

Toxicity: Standard Handling (A)

外形

Lyophilized from 20 mM ammonium bicarbonate solution, 0.1 mg FAF-BSA.

重悬

We recommend resuspending the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 ?g/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage.

分析说明

Positive Control
A-431 cells or skin tissue

其他说明

Hunter T. 1984.Nature311,414.
Masui, H., et al. 1984.Cancer Res.44,1002.
Reynolds, F. H., Jr., et al. 1981. Nature292,259.
Ushiro, H. and Cohen, S. 1980.J. Biol. Chem.255,8363.
This antibody binds to A431, HeLa, and other human cells but not to rodent cells. This antibody inhibits EGF binding to its receptor and is an antagonist ofin vivoEGF-stimulated tyrosine kinase activity. Also inhibits A431 tumor formation in nude mice. For neutralization studies, use Cat. No. GR01L; Cat. No. GR01 contains azide, which is toxic to cells. Antibody should be titrated for optimal results in individual systems.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	528, monoclonal
形式	lyophilized
不包含	preservative
species reactivity	human
should not react with	rat, mouse
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze
同位素/亚型	IgG2a
运输	ambient
储存温度	2-8℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	nwg
闪点(F)	Not applicable
闪点(C)	Not applicable