Anti-Serotonin (5-HT) Transporter (602-622) Rabbit pAb

半岛bd体育手机客户端 说明

一般描述

This Anti-Serotonin (5-HT) Transporter (602-622) Rabbit pAb is validated for use in Frozen Sections, Immunofluorescence for the detection of Serotonin (5-HT) Transporter (602-622).
Recognizes the serotonin (5-HT) transporter in hypothalamus raphe nuclei and spinal cord.
Rabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the serotonin (5-HT) transporter protein.

免疫原

Rat
a synthetic peptide corresponding to amino acids 602-622 of rat 5-HT transporter

包装

100 μL in Plastic ampoule

应用

Frozen Sections (1:10,000-1:15,000; see application references)
Immunoblotting (see comments)
Immunofluorescence (1:200-1:400)

警告

Toxicity: Standard Handling (A)

外形

Undiluted serum.

重悬

Reconstitute the lyophilized antibody with 100 ?l of sterile distilled H?O. Reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Further dilute with sterile PBS or Tris buffer to dilutions no higher than 1:10.

分析说明

Positive Control
Rat naphe nuclei, hypothalamus, cortex, or spinal cord

其他说明

Antibody specificity was examined in the rat cortex, hypothalamus, raphe nuclei, and spinal cord. Staining is completely eliminated by pretreatment of antibody with Serotonin (5-HT) Transporter Control Peptide (Cat. No. PP87) at a concentration of 5 ?g/ml. Low levels of glutaraldehyde (0.1%-0.3%) may be used in conjunction with paraformaldehyde for tissue perfusion fixation. The specificity of this antibody was also determined by immunoblotting analysis using rat brain extracts of the cortex, hypothalamus, midbrain, and hindbrain, which specifically detected a single band. Antibody should be titrated for optimal results in individual systems.
Zhou, F.C., et al. 1996.Mol. Brain Res.43,267.
Blakely, R.D., et al. 1994.J. Exp. Biol.196,263.
Coccaro, E.F. and Murphy, D.L. (Eds.) 1990. inSerotonin in Major Disorders. The Progress in Psychiatry Series, American Psychiatric Press, Washington, D.C.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	serum
antibody product type	primary antibodies
克隆	polyclonal
形式	lyophilized
包含	≤0.1% sodium azide as preservative
species reactivity	mouse, rat
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze avoid repeated freeze/thaw cycles
同位素/亚型	IgG
运输	ambient
储存温度	?20℃

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable