您好,欢迎来到半岛电竞官方网址 ! [ 登录] [ 免费注册]
半岛电竞官方网址
位置: 首页> 品牌> Sigma-Aldrich> Anti-p53 (Ab-11) (Pantropic) Mouse mAb (PAb1802)

Anti-p53 (Ab-11) (Pantropic) Mouse mAb (PAb1802)

品牌
Sigma-Aldrich
货号
OP104L
规格纯度
lyophilized, clone PAb1802, Calbiochem?
价格
6190.47 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
- +
半岛bd体育手机客户端 名称:
Anti-p53 (Ab-11) (Pantropic) Mouse mAb (PAb1802)
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

This Anti-p53 (Ab-11) (Pantropic) Mouse mAb (PAb1802) is validated for use in Immunoblotting, IP, Frozen Sections, IF Paraffin Sections for the detection of p53 (Ab-11) (Pantropic).
Recognizes the ~53 kDa wild-type and mutant p53 protein in immunoblotting. Recognizes only the wild-type p53 protein in immunoprecipitation.
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells. Recognizes the ~53 kDa wild-type and mutant forms of p53.

免疫原

Human
Epitope: within amino acids 306-393 of human p53
recombinant, human p53 protein

包装

100 μg in Plastic ampoule

应用

Immunoblotting (2.5 ?g/ml, chemiluminescence, see application references)

Immunoprecipitation (1 ?g/reaction, see comments, see application references)

Frozen Sections (not recommended)

Immunofluorescence (not recommended)

Paraffin Sections (not recommended)

警告

Toxicity: Standard Handling (A)

外形

Lyophilized from a volatile buffer, 100 ?g BSA.

重悬

Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 ?g/ml; product will be more stable if 0.1% sodium azide is included. Lyophilized antibodies should be reconstituted at 4°C by gradual addition of solvent over a period of 1-2 h with occasional gentle mixing.

分析说明

Positive Control
A431 or SK-BR-3 cells
Negative Control
SK-OV-3 cells

其他说明

Levine, A.J. 1997.Cell88,323.
Miller, S.D., et al. 1997.Mol. Cell. Biol.17,2194.
Pellegata, N.S., et al. 1996.Proc. Natl. Acad. Sci. USA93,15209.
Sturzbecher, H.-W., et al. 1996.EMBO J.15,1992.
Argarwal, M.L., et al. 1995.Proc. Natl. Acad. Sci. USA92,8493.
G?tz, C. and Montenarh, M. 1995.Int. J. Oncology6,1129.
Reed, M. et al. 1995.Proc. Natl. Acad. Sci. USA92,9455.
Waldman, T., et al. 1995.Cancer Res.55,5187.
Clarke, A.R., et al. 1994.Oncogene9,1767.
Cho, Y., et al. 1994.Science265,346.
El-Deiry, W.S., et al. 1994.Cancer Res.54,1169.
Greenblatt, M.S., et al. 1994.Cancer Res.54,4855.
Lane, D.P. 1992.Nature358,15.
This antibody will immunoprecipitate wild-type p53 only. This antibody will stain methanol-fixed cells. Antibody should be titrated for optimal results in individual systems.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平 100
生物来源 mouse
抗体形式 purified antibody
antibody product type primary antibodies
克隆 PAb1802, monoclonal
形式 lyophilized
不包含 preservative
species reactivity human, mouse
manufacturer/tradename Calbiochem?
储存条件 OK to freeze
同位素/亚型 IgG1
运输 ambient
储存温度 2-8℃

安全信息

储存分类代码 11 - Combustible Solids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

Sigma-Aldrich

推荐半岛bd体育手机客户端
Baidu
map