Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421)

半岛bd体育手机客户端 说明

一般描述

Purified mouse monoclonal antibody, originally called clone L21, generated by immunizing mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells (see application references). Recognizes the ~53 kDa wild-type and mutant forms of p53.
Recognizes the ~53 kDa mammalian wild-type and mutant p53 protein. Does not recognize phosphorylated p53.
This Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421) is validated for use in Frozen Sections, Gel Shift, Immunoblotting, IF, FC, IP for the detection of p53 (Ab-1) (Pantropic).

免疫原

partially purified mouse p53
Mouse
Epitope: within amino acids 376-378 of human p53

包装

100 μg in Plastic ampoule

应用

Frozen Sections (10 ?g/ml, see application references)

Gel Shift (see comments)

Immunoblotting (10 ?g/ml, see application references)

Immunofluorescence (1-20 ?g/ml, see application references)

Flow Cytometry (1-20 ?g/ml)

Immunoprecipitation (1 ?g per sample, see application references)

警告

Toxicity: Standard Handling (A)

外形

Lyophilized from a volatile buffer, 100 ?g BSA.

重悬

Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 ?g/ml. Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Store at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide; freezing of aliquots is best for long-term storage of reconstituted product, but repetitive freezing and thawing should be avoided.

分析说明

Negative Control
Normal skin tissue or SK-OV-3 cells
Positive Control
Breast carcinoma, A431 cells, or p53 standard (Cat. No. 506147)

其他说明

Originally called clone L21. Does not recognize phosphorylated or acetylated p53. For a gel shift assay, resuspend in 100 ?l buffer. Wild-type p53 has a short half-life and is present in low amounts in cells. For immunoprecipitation, increasing the amount of sample to be immunoprecipitated and applied to the gel may help visualize wild-type p53, and short incubation times with³⁵S-Met (≤1 h) will help reduce background. For immunoblotting wild-type p53, maximize sensitivity by concentrating samples by immunoprecipitation with Cat. No. OP03, then perform immunoblotting using Cat. No. PC35 and chemiluminescent detection. Antibody should be titrated for optimal results in individual systems.
El-Deiry, W.S., et al. 1994.Cancer Res.54,1169.
Greenblatt, M.S., et al. 1994.Cancer Res.54,4855.
Legros, Y., et al. 1994.Oncogene9,2071.
Barak, Y., et al. 1993.EMBO J.12,461.
Kuerbitz, S.J. 1992.Proc. Natl. Acad. Sci. USA89,7491.
Kastan, M.B., et al. 1992.Cell71,587.
Lane, D.P. 1992.Nature358,15.
Kastan, M.B., et al. 1991.Cancer Res.516304.
Crawford, L. and Harlow, E. 1982.J. Virol.41,709.
Harlow, E., et al. 1981.J. Virol.39861.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	PAb421, monoclonal
形式	lyophilized
不包含	preservative
species reactivity	monkey, rabbit, mouse, human, rat
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze
同位素/亚型	IgG2a
运输	ambient
储存温度	2-8℃

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 3