Anti-Glucocorticoid Receptor (Ab-2) Mouse mAb (BuGR2)

半岛bd体育手机客户端 说明

一般描述

Recognizes the ~94-97 kDa glucocorticoid receptor in mouse liver tissue.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3-AgX-653 myeloma cells (see application references). Recognizes the ~94-97 kDa glucocorticoid receptor.
Anti-Glucocorticoid Receptor (Ab-2), mouse monoclonal, clone BuGR2, recognizes ~94-97 kDa glucocorticoid receptor in mouse liver. It is validated for WB, ICC, IP & IHC (Frozen & paraffin sections).

免疫原

Rat
partially purified, rat glucocorticoid receptor

包装

100 μg in Plastic ampoule

应用

Frozen Sections (5 g/ml)
Immunoblotting (5 g/ml)
Immunocytochemistry (5 g/ml)
Immunoprecipitation (see comments)
Paraffin Sections (5 g/ml)

警告

Toxicity: Standard Handling (A)

外形

Lyophilized from PBS, pH 7.2.

重悬

Resuspend the lyophilized antibody with 0.1 ml sterile PBS, pH 7.2. Be careful to reconstitute the entire contents of the vial; during shipment and handling, portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.

分析说明

Positive Control
mouse liver extract

其他说明

Does not cross react with androgen, progesterone, estrogen or mineralocorticoid receptors. Enzymatic digestion and immunoblot analysis has shown that this antibody reacts with the undigested 97 kDa glucocorticoid receptor, a 17 kDa DNA-binding trypsin fragment, and a 45 kDa steroid and DNA-binding chymotrypsin fragment. Immunocytochemical localization of the glucocorticoid receptor is observed in the cytoplasm and nucleus, even in the presence of hormone. This antibody has also been reported to work for immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
Hussien, A., et al. 1997.Proc. Natl. Acad. Sci. USA94,1521.
Pratt, W.B. and Toft, D.O., 1997.Endocrine Rev.18306.
Schumacher, M., et al. 1996.Dev. Neurosci.18,6.
Toran-Allerand, C.D. 1996.Dev. Neurosci.18,36.
Weigel, N.L. 1996.Biochem J.319,657.
Jones, K.J. 1994.Ann. N.Y. Acad. Sci.743,141.
Arnold, A.P. 1990.in Hormones, Brain and Behavior in Vertebrates, ed. Balthazart, J. (Karger, Basel), 82.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	BuGR2, monoclonal
形式	lyophilized
包含	≤0.1% sodium azide as preservative
species reactivity	rat, mouse, guinea pig, sheep, rabbit
should not react with	avian, amphibian, primate
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze
同位素/亚型	IgG2a
运输	ambient
储存温度	?20℃

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable