半岛bd体育手机客户端 说明
一般描述
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the ~170 kDa EGF receptor protein.
Anti-EGFR (Ab-5), mouse monoclonal, clone EGFR.1, recognizes the ~170 kDa EGF receptor protein in A431 cells. It is validated for use in FC, IF, IP, and neutralization studies.
Recognizes the ~170 kDa EGF receptor protein in A431 cells.
免疫原
partially purified, human EGFR from A431 cells
Human
包装
100 μg in Plastic ampoule
应用
Flow Cytometry (1-5 g/ml)
Immunofluorescence (1-5 g/ml)
Immunoprecipitation (1-2 g/sample)
Neutralization Studies (1-2 g/ml; see application references)
警告
Toxicity: Standard Handling (A)
外形
Lyophilized from a volatile buffer, 100 ?g BSA.
重悬
We recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 ?g/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h.
分析说明
Negative Control
HL-60 cells
Positive Control
A-431 cells
其他说明
Stains A431, HeLa-5 cells and other human cells but not to rodent cells. It inhibits EGF binding to its receptor and is an antagonist ofin vivoEGF-stimulated tyrosine kinase activity. This antibody does not cross-react to c-neu/erbB-2. Antibody should be titrated for optimal results in individual systems.
Hunter T., 1984.Nature311,414.
Reynolds, F.H., Jr., et al. 1981.Nature292,259.
Ushiro, H. and Cohen, S. 1980.J. Biol. Chem.255,8363.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
半岛bd体育手机客户端 性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | EGFR.1, monoclonal |
| 形式 | lyophilized |
| 不包含 | preservative |
| species reactivity | human |
| should not react with | rat, mouse |
| manufacturer/tradename | Calbiochem? |
| 储存条件 | OK to freeze |
| 同位素/亚型 | IgG2b |
| 运输 | ambient |
| 储存温度 | 2-8℃ |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 3 |
Sigma-Aldrich
m.cnreagent.com
