Anti-Cathepsin B (Ab-1) Mouse mAb (CA10)

半岛bd体育手机客户端 说明

一般描述

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/O-NS1 plasmacytoma cells. Recognizes the ~43 kDa latent and the ~31 kDa active forms of cathepsin B.
Anti-Cathepsin B (Ab-1), mouse monoclonal, clone CA10, recognizes the ~43 kDa latent and ~31 kDa active forms of cathepsin B in HepG2 cells. It is validated for WB, IF, and IP.
Recognizes the ~43 kDa latent and the ~31 kDa active forms of cathepsin B in HepG2 cells.

免疫原

purified human liver cathepsin B
Human
Epitope: within the heavy chain of mature cathepsin B

包装

100 μg in Glass bottle

应用

Immunoblotting (250 ng/ml)

Immunoprecipitation (1 ?g/ml)

Immunofluorescence (see comments)

警告

Toxicity: Standard Handling (A)

外形

Lyophilized from 20 mM ammonium bicabonate buffer, 100 ?g BSA (100 ?g size) or 20 ?g BSA (20 ?g size).

重悬

Resuspend the lyophilized antibody with 1 ml sterile PBS. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Store at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide. Freezing of aliquots is best for long-term storage of reconstituted product. Avoid freeze/thaw cycles.

分析说明

Positive Control
Hep G2 cells

其他说明

Sloane, B.F. 1990.inSeminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1:137-152.
Barret, A.J. 1987.Trends Biochem. Sci.12, 193.
Chan, S.J., et al. 1986.Proc. Natl. Acad. Sci. USA83, 7721.
Barrett, A.J. and Kirschke, H. 1981.Meth. Enzymol.80,535.
This antibody recognizes an epitope on the heavy chain of mature cathepsin B and therefore detects both mature and pro-cathepsin B. Also reported to work for immunofluorescence. Antibody should be titrated for optimal results in individual systems.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	CA10, monoclonal
形式	lyophilized
不包含	preservative
species reactivity	human, bovine, porcine
should not react with	rat
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze
同位素/亚型	IgG2a
运输	ambient
储存温度	2-8℃

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable