Anti-c-ErbB2/c-Neu (Ab-3) Mouse mAb (3B5)

半岛bd体育手机客户端 说明

一般描述

Anti-c-ErbB2/c-Neu (Ab-3), mouse monoclonal, clone 3B5, recognizes the ~185 kDa c-ErbB2/c-Neu protein in HEK293 cells. It is validated for WB, IF, IP, and IHC on frozen and paraffin sections.
Recognizes the ~185 kDa c-ErbB2/c-Neu protein in HEK293 cells.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing with Sp2/0.Ag14 cells (see application references). Recognizes the ~185 kDa c-ErbB2/c-Neu protein.

免疫原

a synthetic peptide (TAENPEYLGLDVPV) corresponding to amino acids 1242-1255 from the C-terminal domain of human c-ErbB2/c-Neu
Human

包装

100 μg in Glass bottle

应用

Frozen Sections (2.5 g/ml)
Immunoblotting (2.5 g/ml, see application references)
Immunofluorescence (2.5 g/ml)
Immunoprecipitation (1 g per sample, see application references)
Paraffin Sections (2.5 g/ml, pressure cooker pre-treatment required, see application references)

警告

Toxicity: Standard Handling (A)

外形

Lyophilized from 20 mM ammonium bicarbonate Buffer, 100 ?g FAF-BSA.

重悬

Reconstitue the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 ?g/ml. Lyophilized antibodies should be reconstituted at 4°C with occasional gentle mixing for at least 2 h. Store at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide. Freezing of aliquots is best for long term storage of reconstituted product; repetitive freezing and thawing should be avoided.

分析说明

Positive Control
SK-BR-3 cells or breast carcinoma tissue
Negative Control
HepG2 cells or normal skin

其他说明

Mamcs, J.R., et al. 1994.Ann. Surg.219,332.
Tetu, B., et al. 1994.Cancer73,2359.
DiFiore, P.P., et al. 1987.Science237,178.
Slamon, D.J., et al. 1987.Science235,177.
Varley, J.M., et al. 1987.Oncogene1,423.
Bargmann, C.I., et al. 1986.Nature319,226.
Yamamoto, T., et al. 1986.Nature319,230.
Blick, M., et al. 1984.Blood64,1234.
Schwab, M., et al. 1984.Cold Spring Harbor Laboratory2,215.
For paraffin sections, we recommend pre-treating with a pressure cooker, but trypsin and heat will also work well. Including 0.05% saponin during staining of paraffin sections may help reduce background. In one study ductal carcinomasin situdisplaying large-cell, comedo growth type, were stained while none of 16 ductal carcinomasin situof small-cell, papillary, or cribriform growth type were stained by this antibody. Antibody should be titrated for optimal results in individual systems.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	3B5, monoclonal
形式	lyophilized
不包含	preservative
species reactivity	mouse, human
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze
同位素/亚型	IgG1
运输	ambient
储存温度	2-8℃

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 3