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位置: 首页> 品牌> Sigma-Aldrich> Anti-Eosinophil Major Basic Protein Antibody, clone BMK13

Anti-Eosinophil Major Basic Protein Antibody, clone BMK13

品牌
Sigma-Aldrich
货号
CBL419
规格纯度
clone BMK13, Chemicon?, from mouse
价格
4345.35 *本价格含增值税费
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数量
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半岛bd体育手机客户端 名称:
Anti-Eosinophil Major Basic Protein Antibody, clone BMK13
EMBP
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

特异性

CBL419 is specific for eosinophil major basic protein irrespective of the stages of eosinophil activation and can be regarded as a "pan-eosinophil" marker. This antibody will recognize the 23.8 kDa secreated pro-form of MBP (Pregnancy Associated MBP) found in the placenta and serum of pregnant women as well as the 13.8 kDa mature form of the protein which is found in the matrix of the eosinophil large specific granual. It does not cross-react with horse, mouse or rat MBP.

应用

Immunohistochemistry: Clone BMK-13 reacts with fresh frozen tissues that were sectioned (6μm), dried for 1 hour at room temperature and fixed in 1:1 solution of methanol and acetone, and airdried for 1 hour. Sections can be stored wrapped in aluminium foil at -70°C until used. For paraffin embedded sections, 4% PFA (3hr) should be used for skin biopsies. 5 μM sections are dewaxed, and rehydrated with water. A proteinase treatment of 0.1% trypsin for 15 minutes in water should be performed prior to blocking and antibody staining.The antibody reacts better in frozen tissues than in paraffin embedded tissues.
Western blotting: purified normal human eosinophils were treated with digitonin (5mg/mL) for 10 minutes before boiling in sample buffer and run on a 15% SDS-PAGE. Purified MBP migrates at ~ 14kDa, and typical human samples range from 10-16kDa.
ELISA development for MBP estimation.

Optimal working dilutions must be determined by the end user.
Research Sub Category
Immunoglobulins & Immunology
Anti-Eosinophil Major Basic Protein Antibody, clone BMK13 is an antibody against Eosinophil Major Basic Protein for use in ELISA, WB, IH(P).
Research Category
Inflammation & Immunology

外形

The monoclonal is presented at a concentration of 100μg/ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.
Format: Purified

储存及稳定性

For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

质量水平 100
生物来源 mouse
抗体形式 purified antibody
antibody product type primary antibodies
克隆 BMK13, monoclonal
species reactivity human
should not react with rat, mouse, horse
manufacturer/tradename Chemicon?
technique(s) ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
同位素/亚型 IgG1
NCBI登记号 NM_002728.4
UniProt登记号 P13727
运输 wet ice
Gene Information human ... PRG2(5553)

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 2
闪点(F) Not applicable
闪点(C) Not applicable

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