Rat/Mouse Ghrelin (total) ELISA

半岛bd体育手机客户端 说明

一般描述

Circulating ghrelin is a multifunctional hormone secreted primarily by the stomach. Originally found as an endogenous ligand for the growth hormone secretagogue receptor, it is also an appetite-stimulating hormone in the regulation of energy homeostasis. It consists of 28 amino acids and the n-octanoylation of serine-3 position is necessary for its bioactivity. The acylated form is extremely unstable in the blood; consequently, the predominant form is des-octanoyl ghrelin. Long considered a key player in obesity, ghrelin may also play a role in maintaining the balance between insulin and glucose and the development of diabetes. The spike in insulin secretion occurring after eating usually correlates with a dip in ghrelin production. Researchers determined that in rat models of uncontrolled diabetes, food intake still suppressed ghrelin concentrations in the blood, but only approximately half as effectively as when insulin was present.

特异性

Canine Ghrelin (Active) - 71%
Human Ghrelin (Active) - 115%
Human Des-Octanoyl Ghrelin - 249%
Porcine Ghrelin (Active) - 48%
Rat/Mouse Ghrelin (Active) - 85%
Rat/Mouse Des-Octanoyl Ghrelin - 100%

应用

Room temperature, 3 hour assay, 20 μL sample size (serum or plasma)
Research Sub Category
Obesity

Metabolic Disorders
Research Category
Metabolism
Used to detect/quantify: Ghrelin (Total)

其他说明

Please contact Technical Service for linearity of dilution.

免责声明

For research use only. Not for use in diagnostic procedures.

基本信息

eCl@ss	32161000
NACRES	NA.32

半岛bd体育手机客户端 性质

质量水平	100
species reactivity	rat, mouse
assay range	accuracy: 80-103% (Serum: Active Ghrelin) accuracy: 86-102% (Plasma: Active Ghrelin) accuracy: 92-103% (Serum: Des-octanoyl Ghrelin) accuracy: 95-107% (Plasma: Des-octanoyl Ghrelin) inter-assay cv: 1.8-4.5% intra-assay cv: 0.7-1.3% (Serum) inter-assay cv: 2.0-3.2% intra-assay cv: 1.1-1.7% (Plasma)
technique(s)	ELISA: suitable
UniProt登记号	Q9EQX0 (mouse) Q9QYH7 (rat) Q9UBU3
detection method	colorimetric fluorometric

安全信息

象形图
警示用语：	Danger
危险声明	H290 - H302 + H332 - H311 - H315 - H317 - H319 - H335 - H411
预防措施声明	P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338
危险分类	Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
靶器官	Respiratory system
储存分类代码	6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK	WGK 3