半岛bd体育手机客户端 说明

一般描述

α-Ketoglutarate dehydrogenase (α-KGDH) is a multienzyme complex localized to the mitochondria. This integrated enzyme is made up of many units of thiamine pyrophosphate-dependent dehydrogenase (E1), dihydrolipoamide dehydrogenase (E3), and dihydrolipoamide succinyl transferase (E2).

应用

α-Ketoglutarate Dehydrogenase from the porcine heart has been used:

• to study the reversal of nitration by glutathione (GSH) in peroxynitrite-treated cells
• to measure its activity by Spectramax M5 microplate spectrofluorimeter using heart mitochondria
• as a positive control to evaluate its activity in by Spectramax GEMINI EM fluorescence microplate reader using mice neurons

生化/生理作用

α-Ketoglutarate dehydrogenase (α-KGDH) is a key enzyme of bioenergetic processes and a controlling unit of metabolic flux through the Krebs cycle or tricarboxylic acid (TCA) cycle. It catalyzes the oxidative decarboxylation of α-ketoglutarate (KG) to succinyl-CoA by releasing reduced nicotinamide adenine dinucleotide (NADH). It is the rate-limiting reaction of the TCA cycle. This reaction contributes to the electrons of the respiratory chain and requires thiamine pyrophosphate as a cofactor. The reduction of NAD (nicotinamide adenine dinucleotide) is observed to determine its reaction rate. α-KGDH from porcine has an optimum pH range of 6.6–7.4. This enzyme is inhibited by oxidative stress and results in a metabolic deficiency. However, α-KGDH is also known to produce reactive oxygen species (ROS) leading to oxidative stress. Defective or limited levels of α-KGDH cause several neurodegenerative diseases such as Alzheimer′s disease.

质量

可能含有痕量的聚乙二醇。

单位定义

在 30 ℃、pH 7.4、饱和水平的辅酶 A 存在的条件下，一单位每分钟可将 1.0 μ 摩尔的 β-NAD 转化成 β-NADH。

外形

以 50％ 甘油溶液的形式提供，含有 9mg 每毫升的牛血清白蛋白、30％ 蔗糖、1.5 mM EDTA、1.5 mM EGTA、1.5 mM 2-巯基乙醇、0.3％ TRITON^?X-100、0.003％ 叠氮化钠和 15 mM 磷酸钾，pH 6.8。

法律信息

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

基本信息

酶学委员会(EC)编号	1.2.4.2 ( BRENDA | IUBMB )
MDL编号	MFCD00130490
NACRES	NA.54

半岛bd体育手机客户端 性质

形式	buffered aqueous glycerol solution
质量水平	200
specific activity	0.1-1.0 units/mg protein (Lowry)
异质活性	pyruvate dehydrogenase ≤20%
运输	dry ice
储存温度	?20℃

安全信息

危险声明	H412
预防措施声明	P273 - P501
危险分类	Aquatic Chronic 3
储存分类代码	10 - Combustible liquids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)