半岛bd体育手机客户端 说明
一般描述
The bacteriophage MS2 is a small, male-specific RNA bacteriophage that infects E. coli and other members of the Enterobacteriaceae family. Its genome comprises a single sense (+) strand of RNA 3569 nucleotides in length, which encodes only four proteins: the coat protein, the A or maturation protein, which is required for infectivity, a subunit of the viral replicase, and a small lysis protein encoded in an overlapping reading frame. The capsid protein (UniProt: P03612; also known as CP, Coat protein) of bacteriophage MS2 is a translational repressor. It is reported to inhibit the synthesis of the viral replicase by binding a specific RNA structure that contains the replicase translation initiation region. Capsid protein self-assembles to form an icosahedral capsid with a T=3 symmetry and consists of 89 capsid proteins dimers (178 capsid proteins). It is involved in viral genome encapsidation through the interaction between a capsid protein homodimer and the multiple packaging signals present in the RNA genome. Proline 78 is shown to be essential for viral infectivity. (Ref.: Hill, HR et al. (1997). J. Mol. Biol. 266(1); 1-7).
特异性
This rabbit polyclonal antibody detects MS2 bacteriophage capsid protein. The immunogen used has an N- terminus His tag and mutations to avoid dimerization and capsid formation.
免疫原
His-tagged recombinant Enterobacteriophage MS2 Coat Protein (aa 2-130).
应用
Anti-EnterobacterioPhage MS2 Coat Protein, Cat. No. ABE76-I, is a highly specific rabbit polyclonal antibody that targets Escherichia phage MS2 (Bacteriophage MS2) Capsid Protein and has been tested in Immunoprecipitation and Western Blotting.
Research Category
Epigenetics & Nuclear Function
Western Blotting Analysis: A 1:5,000 dilution from a representative lot detected recombinant EnterobacterioPhage MS2 Coat Protein and wildtype MS2 protein (Courtesy of Caroline Horizny from CNSE at SUNY Albany, Albany, NY).
Immunoprecipitation Analysis: A 1:10,000 dilution from a representative lot detected EnterobacterioPhage MS2 Coat Protein in K562 cells treated with Escherichia phage MS2 (Bacteriophage MS2).
质量
Evaluated by Western Blotting in K562 treated with Escherichia phage MS2 (Bacteriophage MS2) and K562+MS2 recombinant protein treated with Escherichia phage MS2 (Bacteriophage MS2) Capsid Protein.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected EnterobacterioPhage MS2 Coat Protein in K562 ells treated with Escherichia phage MS2 (Bacteriophage MS2) and K562+MS2 recombinant protein treated with Escherichia phage MS2 (Bacteriophage MS2) Capsid Protein.
目标描述
~14 kDa observed; 13.86 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
外形
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity Purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
其他说明
Concentration: Please refer to lot specific datasheet.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
半岛bd体育手机客户端 性质
| 生物来源 | rabbit |
| 质量水平 | 100 |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 纯化方式 | affinity chromatography |
| species reactivity | virus |
| species reactivity (predicted by homology) | all |
| 包装 | antibody small pack of 25 μg |
| technique(s) | immunoprecipitation (IP): suitable western blot: suitable |
| NCBI登记号 | NP_040648.1 |
| UniProt登记号 | P03612 |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
