Monoclonal Anti-ILK antibody produced in mouse

半岛bd体育手机客户端 说明

一般描述

Monoclonal Anti-ILK (mouse IgG2b isotype) is derived from the 65.1 hybridoma produced by the fusion of mouse myeloma cells (P3X63-Ag8.653) and splenocytes from BALB/c mice immunized with purified mouse ILK recombinant protein. Integrin-linked kinase (ILK) is a ubiquitously expressed 50-59 kDa serine/threonine kinase that has three structurally well-conserved domains. A C-terminal domain contains the kinase catalytic site as well as the binding site for integrin β1 cytoplasmic domain. A N-terminal domain contains four ankyrin repeats (ANK).

免疫原

purified mouse ILK recombinant protein.

应用

Monoclonal Anti-ILK antibody produced in mouse has been used in:

• immunostaining
• immunoprecipitation
• immunocytochemistry
• immunohistochemistry
• western blotting

包装

200 μL in poly bottle

生化/生理作用

Integrin-linked kinase (ILK) is a serine-threonine kinase that interacts with PINCH and parvin to modulate cell adhesion, growth, differentiation, migration and invasion. This kinase binds to integrin β1, β2 and β3 domains to regulate integrin signalling. ILK functions as a receptor-proximal effector for integrin and growth factor dependent signal transduction. ILK is associated with cell cycle progression and oncogenic transformation. The kinase activity of ILK is low in non-activated cells; its activity is stimulated by cell-extracellular matrix (ECM) interactions and by certain growth factors. Negative regulation of ILK is mediated by two phosphatases: phosphatase and tensin homolog (PTEN), a tumor suppressor lipid phosphatase and ILKAP (ILK associated serine/threonine phosphatase), a protein phosphatase 2C (PP2C) protein phosphatase. In tumor cells that do not express PTEN protein, ILK is constitutively active.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号	MFCD01633545
NACRES	NA.44

半岛bd体育手机客户端 性质

质量水平	200
生物来源	mouse
偶联物	unconjugated
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	65.1, monoclonal
形式	buffered aqueous solution
分子量	antigen ~59 kDa
species reactivity	human, canine, mouse, monkey, bovine, rat
浓度	~2 mg/mL
technique(s)	immunocytochemistry: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable microarray: suitable western blot: 0.5-2.0 μg/mL using whole cell extract of Chinese hamster ovary cell line (CHO cells)
同位素/亚型	IgG2b
UniProt登记号	Q13418
运输	dry ice
储存温度	?20℃
Gene Information	human ... ILK(3611) mouse ... Ilk(16202) rat ... Ilk(170922)