半岛bd体育手机客户端 说明

一般描述

Isopropyl thiogalactose (IPTG) is a sugar derivative widely used for the induction of the expression of recombinant proteins inE. coli. This expression system requires that the gene of interest is cloned in plasmids containing the Lac promoter. IPTG Ready Made is a 0.2 μm filter sterilized solution of 200 mM IPTG. This reagent remains liquid at its storage temperature (-20 ℃), thus avoiding freeze-thaw cycles and providing a user-friendly solution.

应用

Isopropyl β- D -thiogalactopyranoside solution is:

• used as an inducer for the expression of single domain antibodies(sdAbs) inE coliand bioactive peptides
• used in its half-life and decay studies
• suitable for induction of recombinant protein expression in systems controlled by the lac repressor

生化/生理作用

Isopropyl β-D-thiogalactopyranoside binds to the lac repressor and releases the tetrameric repressor from the lac operator in an allosteric manner. This binding allows the transcription of genes in the lac operon, such as the gene coding for β--galactosidase. The β-galactosidase gene is transcribed and monosaccharides are the product. Since Isopropyl β-D-thiogalactopyranoside cannot be metabolized byE. coliits concentration remains constant. Therefore, it is not a variable in the experiment.

组分

IPTG Solution is composed of 200 mM IPTG in molecular biology grade water.

原理

Isopropyl β-D-thiogalactopyranoside binds to the lac repressor and releases the tetrameric repressor from the lac operator in an allosteric manner. This binding allows the transcription of genes in the lac operon, such as the gene coding for β-galactosidase. The β-galactosidase gene is transcribed and monosaccharides are the product.

基本信息

经验(实验)分子式	C9H18O5S
分子量	238.30
Beilstein	4631
MDL编号	MFCD00063273
PubChem化学物质编号	24895928
NACRES	NA.52

半岛bd体育手机客户端 性质

质量水平	200
等级	for molecular biology
描述	non-ionic
形式	liquid
运输	dry ice
储存温度	?20℃
SMILES string	CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O
InChI	1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1
InChI key	BPHPUYQFMNQIOC-NXRLNHOXSA-N

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)