半岛bd体育手机客户端 说明
一般描述
Inositol-requiring enzyme-1 (IRE1), consists of an N-terminal endoplasmic reticulum (ER) luminal domain, a transmembrane domain, and a C-terminal cytoplasmic region composed of a Ser/Thr protein kinase domain and a site-specific endoribonuclease (RNase) domain.
The gene ERN1 (endoplasmic reticulum to nucleus signaling 1) encodes an ER transmembrane kinase/endoribonuclease that is also referred to as IRE1α (inositol-requiring transmembrane kinase and endonuclease 1α). The serine-threonine protein kinase encoded by this gene is ubiquitously expressed in cells and tissues.
应用
Anti-IRE1α antibody produced in rabbit has been used in western blotting and immunoprecipitation.
生化/生理作用
ER transmembrane kinase/endoribonuclease serves as an ER stress sensor that initiates the splicing of the mRNA encoding X-box–binding protein 1 (XBP-1) upon activation. Spliced XBP-1 mRNA produces homeostatic transcription factor XBP1. Under ER stress, the RNase activity of IRE1α cleaves many ER-localized mRNAs associated with apoptosis. The cytosolic motif of the activated form of IRE1α associates with the adaptor protein TRAF2 (tumor necrosis factor (TNF)–associated factor 2). This complex activates the c-Jun N-terminal kinase (JNK) signaling pathway.
Inositol-requiring enzyme-1 (IRE1) is involved in the unfolded protein response (UPR), a transcriptional program induced by endoplasmic reticulum (ER) stress. The endonuclease activity of IRE1 autoregulates its mRNA and is required for the UPR. IRE1 senses the status of luminal protein folding in the ER via its N-terminal luminal domain. Presence of unfolded and misfolded proteins leads to dimerization, trans-autophosphorylation and activation of IRE1.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.41 |
半岛bd体育手机客户端 性质
| 生物来源 | rabbit |
| 质量水平 | 200 |
| 偶联物 | unconjugated |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~110 kDa |
| species reactivity | human |
| 浓度 | ~1 mg/mL |
| technique(s) | immunoprecipitation (IP): 2-5 μg using lysate of HEk-293T cells expressing human IRE1α indirect immunofluorescence: suitable western blot: 1-2 μg/mL using whole extract of HEK-293T cells expressing human IRE1α |
| UniProt登记号 | O75460 |
| 运输 | dry ice |
| 储存温度 | ?20℃ |
| Gene Information | human ... ERN1(2081) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
Sigma-Aldrich
m.cnreagent.com
