半岛bd体育手机客户端 说明
一般描述
Recombinant human Interleukin-4 (IL-4) is expressed in human 293 cells as a glycoprotein with a calculated moleculaer mass of 14.9 kDa. This protein is manufactured in human cells using an all-human production system, with full chemically defined ingredients and with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports better stability of the protein in culture.
生化/生理作用
IL-4 is a protein that has been shown to regulate a wide spectrum of functions in B cells, T cells, monocytes/macrophages and other haematopoietic and non-haematopoietic cells. Among T cell clones, IL-4 is produced by Th0 and Th2 cells, but not Th1 cells, and this has now been demonstrated both in mice and in humans. IL-4 blocks the production of proinflammatory cytokines such IL-6, TNFα, and M-CSF and improves the differentiation of immature DCs from CD34+ progenitors when added during the differentiation period (day 6 to day 12).
Interleukin-4 is a lymphokine with profound effects on the growth and differentiation of immunologically competent cells. IL-4 is also known as B cell stimulatory factor-1 (BSF-1), T cell growth factor-2 (TCGF-2) and mast-cell growth factor-2 (MCGF-2). Inhibits VEGF-induced and bFGF-induced angiogenesis. IL-4 is a complex glycoprotein released by a subset of activated T cells. Human and mouse IL-4 share 50% amino acid sequence homology, but their biological actions are species-specific.
序列
HKCDITLQEIIKTLNSLTEQKTLCTELTVTDIFAASKNTTEKETFCRAATVLRQFYSHHEKDTRCLGATAQQFHRHKQLIRFLKRLDRNLWGLAGLNSCPVKEANQSTLENFLERLKTIMREKYSKCSS
外形
Supplied as a lyophilized powder containing phosphate buffered saline.
分析说明
The biological activity of recombinant human IL-4 was tested in culture by measuring its ability to stimulate proliferation of human TF-1 cells (human erythroleukemic indicator cell line).
基本信息
| MDL编号 | MFCD00151705 |
| NACRES | NA.77 |
半岛bd体育手机客户端 性质
| 重组 | expressed in HEK 293 cells |
| 质量水平 | 200 |
| 测定 | ≥98% (SDS-PAGE) |
| 效能 | ≤1 ng/mL ED50 |
| 分子量 | 14.9 kDa (glycosylated) |
| technique(s) | cell culture | mammalian: suitable |
| 适用性 | endotoxin tested |
| 储存温度 | ?20℃ |
安全信息
| 危险声明 | H412 |
| 预防措施声明 | P273 - P501 |
| 危险分类 | Aquatic Chronic 3 |
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
