Anti-SH3KBPI Antibody, clone 179.1.E1

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一般描述

SH3 domain-containing kinase-binding protein 1 (UniProt: Q925Q9; also known as Regulator of ubiquitous kinase, Ruk, SH3-containing expressed in tumorigenic astrocytes, SH3KBPI,) is encoded by the Sh3kbp1 (also known as Ruk, Seta) gene (Gene ID: 84357) in rat. SH3KBPI is a peripheral membrane protein that serves as an adapter protein involved in regulating diverse signal transduction pathways. It localizes in endocytic vesicles containing clustered receptors. It is shown to be highly expressed in the spinal cord. It can self-associate and form homotetramers. It contains three SH3 domains (aa 1-58; 98-157; and 311-372). SH3KBPI is involved in the regulation of endocytosis and lysosomal degradation of ligand-induced receptor tyrosine kinases, including EGFR and MET/hepatocyte growth factor receptor, through an association with CBL and endophilins. It can attenuate phosphatidylinositol 3-kinase activity by interaction with its regulatory subunit. It is also reported to plays a role in the regulation of cell morphology and cytoskeletal organization. Seven different isoforms of SH3KBP1 have been described that are produced by alternative splicing.

特异性

Clone 179.1.E1 is a mouse monoclonal antibody that specifically detects SH3 domain-containing kinase-binding protein 1.

免疫原

His-tagged full-length recombinant rat SH3 domain-containing kinase-binding protein 1.

应用

Anti-SH3KBPI, clone 179.1.E1, Cat. No. 05-731-I, is a mouse monoclonal antibody that detects SH3 domain-containing kinase-binding protein 1 and has tested for use in Western Blotting.
Western Blotting Analysis: A representative lot from this antibody detected SH3KBPI in NG108-15 cell lysate.

Research Category
Apoptosis & Cancer

质量

Evaluated by Western Blotting in THP-1 cell lysate.

Western Blotting Analysis: 0.5 ?g/mL of this antibody detected SH3KBPI in THP-1 cell lysate.

目标描述

~85 kDa observed; 78.09 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Protein G purified
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss

32160702

半岛bd体育手机客户端 性质

生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	179.1.E1, monoclonal
species reactivity	human, rat, mouse
包装	antibody small pack of 25 μg
technique(s)	western blot: suitable
同位素/亚型	IgG2bκ
NCBI登记号	NP_445812
UniProt登记号	Q925Q9