Anti-IL-22RA-1 Antibody

半岛bd体育手机客户端 说明

一般描述

Interleukin-22 receptor subunit alpha-1 (UniProt Q8N6P7; also known as CRF2-9, Cytokine receptor class-II member 9, Cytokine receptor family 2 member 9, IL-22 receptor subunit alpha-1, IL-22R-alpha-1, IL-22RA1, ZcytoR11) is encoded by the IL22RA1 (also known as IL22R) gene (Gene ID 58985) in human. IL-22RA-1 is a member of the type II cytokine receptor family that complexes with IL-10RB and mediates JAK/STAT signaling. IL22 binding to heterodimer is greater than binding to IL22RA1 alone. IL-22RA-1 is expressed in colon, liver, lung, pancreas and kidney, however, no expression is observed in immune cells, such as monocytes, T-cells, and NK-cells. It is also detected in normal blood brain barrier endothelial cells as well as in multiple sclerosis lesions. Higher expression has been observed in synovial fluid cells from rheumatoid arthritis and spondyloarthropathy patients.

特异性

This polyclonal antibody targets a C-terminal sequence within the cytoplasmic domain of human IL-22 receptor subunit alpha-1.

免疫原

KLH-conjugated linear peptide corresponding to a sequence within the C-terminal region of human IL-22RA-1 cytoplasmic domain.

应用

This rabbit polyclonal Anti-IL-22RA-1 Antibody, Cat. No. 06-1077-I is validated for use in Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting for the detection of Interleukin-22 receptor subunit alpha-1.
Flow Cytometry Analysis: 1 ?g from a representative lot detected IL-22RA-1 immunoreactivity among one million 4% paraformaldehyde-fixed, 0.2% Triton X-100-permeabilized SW480 human colon cancer cells.

Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected IL-22RA-1 in human tonsil and prostate tissue sections.

Research Category
Inflammation & Immunology

质量

Evaluated by Western Blotting in mouse spleen tissue lysate.

Western Blotting Analysis: 2 ?g/mL of this antibody detected IL-22RA-1 in 10 ?g of mouse spleen tissue lysate.

目标描述

~63 kDa observed. 61.54 kDa (signal peptide a.a. 1-15 removed) calculated. Uncharacterized bands may be observed in some lysate(s).

联系

Replaces: 06-1077

外形

Affinity purified.
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss

32160702

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	human, mouse
technique(s)	flow cytometry: suitable immunohistochemistry: suitable (paraffin) western blot: suitable
NCBI登记号	NP_067081
UniProt登记号	Q8N6P7
运输	ambient
Gene Information	human ... IL22RA1(58985)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable