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位置: 首页> 品牌> Sigma-Aldrich> Anti-Ubiquityl-Histone H2B Antibody, clone 56

Anti-Ubiquityl-Histone H2B Antibody, clone 56

品牌
Sigma-Aldrich
货号
05-1312-I
规格纯度
clone 56, from mouse
价格
7197.66 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
- +
半岛bd体育手机客户端 名称:
Anti-Ubiquityl-Histone H2B Antibody, clone 56
Histone H2B type 1-C/E/F/G/I, Histone H2B.1 A, Histone H2B.a, H2B/al, Histone H2B.g, H2B/g, Histone H2B.h, H2B/h, Histone H2B.k, H2B/k, Histone H2B.l, H2B/l)
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Histone H2B type 1-C/E/F/G/I (UniProt: P62807; also known as Histone H2B.1 A, Histone H2B.a, H2B/a, Histone H2B.g, H2B/g, Histone H2B.h, H2B/h, Histone H2B.k, H2B/k, Histone H2B.l, H2B/l) is encoded by the HIST1H2BC (also known as H2BFL, HIST1H2BE H2BFH, HIST1H2BF, H2BFG, HIST1H2BG, H2BFA, HIST1H2BI, H2BFK) gene (Gene ID: 3017, 8339, 8343, 8344, 8346, 8347) in human. Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. Histone modifications regulate DNA transcription, repair, recombination, and replication. Histone H2B is one of the core components of the nucleosome, the smallest subunit of chromatin. DNA base pairs are wrapped around an octamer of core histone proteins consisting of two each of Histone H2A, H2B, H3 and H34. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. A number of studies have implicated ubiquitinated H2B in heterochromatic gene silencing and in the control of gene expression. Histone H2B is ubiquitylated at Lys120 by RAD6 E2 protein in conjunction with RNF20/RNF40 and this ubiquitinylated form is found preferentially associated with the transcribed regions of highly expressed genes.

特异性

Clone 56 recognizes histone H2B when ubiquitinated on Lys120. It does not recognize H2B in its unmodified state or ubiquitin unconjugated to H2B.

免疫原

A branched peptide corresponding to the ubiquitination site on human H2B.

应用

Anti-Ubiquityl-Histone H2B, clone 56 Antibody, Cat. No. 05-1312-I, is a highly specific mouse monoclonal antibody that targets Ubiquityl-Histone H2B and has been tested in Chromatin Immunoprecipitation (ChIP) and Western Blotting.
Research Category
Epigenetics & Nuclear Function
Western Blotting Analysis: A representative lot detected Ubiquityl-Histone H2B in HeLa, NIH3T3, and PC-12 cell lysate.

Western Blotting Analysis: A representative lot detected Ubiquityl-Histone H2B in U2OS whole cell extract, purified histone H2A, purified H2B, and HEK293 acid extracted proteins (Courtesy of Dr. Moshe Oren, Weizmann Institute of Science).

Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Ubiquityl-Histone H2B in uH2B and H3K4me2 (Courtesy of Dr. Moshe Oren, Weizmann Institute of Science).

质量

Evaluated by Western Blotting in PC-12 cell lysate.

Western Blotting Analysis: 0.25 ?g/mL of this antibody detected Ubiquityl-Histone H2B in 10 ?g of PC-12 cell lysate.

目标描述

~25 kDa observed. Uncharacterized bands may be observed in some lysate(s).

外形

Protein G purified
Format: Purified
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

质量水平 100
生物来源 mouse
抗体形式 purified antibody
antibody product type primary antibodies
克隆 56, monoclonal
species reactivity rat, human, mouse
包装 antibody small pack of 25 μg
technique(s) ChIP: suitable
western blot: suitable
同位素/亚型 IgG2aκ
NCBI登记号 NP_066406.1
UniProt登记号 P62807
运输 ambient
Gene Information human ... HIST1H2BC(8347)

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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