Anti-Prohormone Convertase PC2 Antibody

半岛bd体育手机客户端 说明

一般描述

Neuroendocrine convertase 2 (EC 3.4.21.94; UniProt P16519; also known as KEX2-like endoprotease 2, NEC 2, PC2, Prohormone convertase 2, Proprotein convertase 2) is encoded by the PCSK2 (also known as NEC2) gene (Gene ID 5126) in human. Neuroendocrine convertase 2/PC2 belongs to the family of subtilisin-related proteolytic enzymes involved in the proteolytic cleavages of peptide hormone precursors. For example, during the multistep processing of the peptide precursor proopiomelanocortin (POMC), PC2 catalyzes the cleavage of adrenocorticotropic hormone 1–39 (ACTH) into adrenocorticotropic hormone 1–14, which is then further processed to produce α-melanocyte-stimulating hormone (α-MSH). PC2 is produced initially in a prepro-form with a signal peptide (a.a. 1-25) and a propeptide (a.a. 26-109) sequence, the removal of which yields the mature 529-amino acid enzyme (a.a. 110-638). The neuroendocrine protein 7B2 blocks proPC2 from forming unactivatable aggregates and is absolutely required for processing proCP2 into active enzyme. Newly synthesized proPC2 is N-glycosylated in the ER and undergoes rate-limiting folding process before it can bind 7B2 and be transported to the TGN/ secretory granules for maturation by sulfation and proteolytic cleavage.

特异性

This polyclonal antibody detects the ~68 kDa secreted mature PC2 in mouse cerebellum lysate by Western blotting. Expected to recognize also the Prepro- and pre-forms of all three spliced human isoforms reported by UniProt (P16519).

免疫原

Epitope: Near the N-terminus of mature PC2.
GST-tagged recombinant human mature PC2 N-terminal fragment.

应用

Immunohistochemistry Analysis: An 1:1,000 dilution from a representative lot detected Prohormone Convertase PC2 in human cerebral cortex, pancreas and testis tissue sections.
Research Sub Category
Hormones & Receptors
Anti-Prohormone Convertase PC2 Antibody is an antibody against Prohormone Convertase PC2 for use in Western Blotting, Immunohistochemistry.
Research Category
Neuroscience

质量

Evaluated by Western Blotting in mouse cerebellum tissue lysate.

Western Blotting Analysis: 0.5 ?g/mL of this antibody detected Prohormone Convertase PC2 in 10 ?g of mouse cerebellum tissue lysate.

目标描述

~68 kDa calculated. Target band appears larger than the calculated molecular weight of the mature form (58.28 kDa/mouse & 58.00 kDa/human) due to posttranslational glycosylation and sulfation. 67.97/63.72/68.12 kDa (human isoform 1/2/3 proforms), 70.57/66.31/68.58 kDa (human isoform 1/2/3 prepro-forms) calculated.

联系

Replaces: AB15610

外形

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	mouse, human
species reactivity (predicted by homology)	porcine (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology)
technique(s)	immunohistochemistry: suitable western blot: suitable
NCBI登记号	NP_002585
UniProt登记号	P16519
运输	wet ice
Gene Information	human ... PCSK2(5126)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable