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Fas Ligand human

品牌
Sigma-Aldrich
货号
SRP3036
规格纯度
recombinant, expressed in CHO cells, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture
价格
4582.93 *本价格含增值税费
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数量
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半岛bd体育手机客户端 名称:
Fas Ligand human
Apo I Ligand
CD95L
TNFSF6
soluble Fas Ligand (sFasL)
APTL
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Fas Ligand (FasL) is a member of the TNF (tumor necrosis factor) superfamily that is expressed on the cell surface of activated T cells. It can be present as soluble form in the circulation or membrane bound form in cells. Recombinant human soluble Fas Ligand is a 17.9kDa protein comprising the TNF homologous region of FasL and contains an 8 residue N-terminal His-Tag. Both human and murine sFasL are fully active on human and murine cells.

应用

Fas ligand human has been used to induce apoptosis in T-cell lymphoma-derived HuT78 cells and jurkat cells.

生化/生理作用

Binding of Fas Ligand (FasL) to Fas receptor triggers apoptosis in Fas-bearing cells. FasL has the ability to kill T cells and activated B cells which leads to down-regulation of the immune response. The mechanism of Fas induced apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD (Fas-Associated protein with death domain) followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to the eventual cell death. FasL is also involved in AGE (advanced glycation end-product)-mediated apoptosis in human retinal ARPE-19 cells, suggesting its role in diabetic retinopathy. Changes in the activity of FasL suppresses normal apoptosis, leading to abnormal survival and growth of tumor cells. Mutations in the FasL gene causes autoimmune lymphoproliferative syndrome.

序列

HHHHHHHHPS PPPEKKELRK VAHLTGKSNS RSMPLEWEDT YGIVLLSGVK YKKGGLVINE TGLYFVYSKV YFRGQSCNNL PLSHKVYMRN SKYPQDLVMM EGKMMSYCTT GQMWARSSYL GAVFNLTSAD HLYVNVSELS LVNFEESQTF FGLYKL

外形

Lyophilized with no additives.

重悬

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8℃ for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20℃ to -80℃.

基本信息

NACRES NA.32

半岛bd体育手机客户端 性质

生物来源 human
重组 expressed in CHO cells
测定 ≥95% (HPLC)
≥95% (SDS-PAGE)
形式 lyophilized
效能 ≤10.0 ng/mL
分子量 17.9 kDa
包装 pkg of 10 μg
technique(s) cell culture | mammalian: suitable
杂质 <0.1 EU/μg endotoxin, tested
颜色 white to off-white
UniProt登记号 P48023
运输 wet ice
储存温度 ?20℃
Gene Information human ... FASLG(356)

安全信息

储存分类代码 13 - Non Combustible Solids
WGK WGK 3
闪点(F) Not applicable
闪点(C) Not applicable

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