半岛bd体育手机客户端 说明

一般描述

Anti HA-FITC is a mouse monoclonal antibody derived from the HA-7 hybridoma. It is isolated from ascites fluid and conjugated to fluorescein isothiocyanate, isomer I (FITC). The antibody recognizes the HA tag expressed on the N- or C-terminus on HA tagged H7411proteins. The antibody reacts specifically with HA tagged proteins by immunocytochemistry.
Hemagglutinin (HA) is the major envelope glycoprotein of the influenza virus, along with neuraminidase.

特异性

该抗体可识别HA-标签蛋白的天然和还原变性形式，并且可与大肠杆菌或哺乳动物表达的N-末端或C-末端HA-标签融合蛋白发生反应。

免疫原

与人流感病毒血凝素(HA)片段相对应的合成肽，称为HA-标签，与KLH结合

应用

Antibody suitable for Immunofluorescence. Recommended concentration 1-5 mg/ml. For immunocytochemistry, use HA tagged fusion protein transfected into mammalian cells, then fixed with paraformaldehyde. Anti-HA-FITC antibody, Mouse monoclonal has been used:

• in the labeling of HA-tagged transient receptor potential melastatin 7 (TRPM7) channel protein for immunocytochemistry studies in lymphoblast cell DT40 and human embryonic kidney cells (HEK-293) cells
• in western blotting detection of spindle-associated proteins inTrypanosoma brucei
• in immunostaining of vascular smooth muscle cells (SMCs) for immunocytofluorescence studies

生化/生理作用

Hemagglutinin (HA) induces immune response post viral infection. Fluorescein isothiocyanate (FITC) conjugated with the influenza virus HA is majorly used in intracellular tracking of the virus and also in flow cytometric studies.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide as a preservative.

基本信息

MDL编号	MFCD00803873
NACRES	NA.56

半岛bd体育手机客户端 性质

质量水平	200
生物来源	mouse
偶联物	FITC conjugate
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	HA-7, monoclonal
形式	buffered aqueous solution
储存条件	protect from light
浓度	1 mg/mL
technique(s)	immunocytochemistry: 1-5 μg/mL using HA tagged fusion protein transfected into mammalian cells fixed with paraformaldehyde.
运输	wet ice
储存温度	?20℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable