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位置: 首页> 品牌> Sigma-Aldrich> PhosphoDetect Anti-EGFR (pTyr845) Rabbit pAb

PhosphoDetect Anti-EGFR (pTyr845) Rabbit pAb

品牌
Sigma-Aldrich
货号
324865
规格纯度
liquid, Calbiochem?
价格
8539.21 *本价格含增值税费
促销
服务
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数量
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半岛bd体育手机客户端 名称:
PhosphoDetect Anti-EGFR (pTyr 8 4 5) Rabbit pAb
Anti-Epidermal Growth Factor Receptor (pTyr 8 4 5)
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site and a generic tyrosine phosphorylated peptide, followed by immunoaffinity chromatography. Recognizes the ~185 KDa EGFR protein phosphorylated on Tyr845.
Recognizes the ~170 kDa EGFR protein phosphorylated at Tyr845in EGF-treated A431 cells. Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
This PhosphoDetect Anti-EGFR (pTyr???) Rabbit pAb is validated for use in Immunoblotting for the detection of EGFR (pTyr???).

免疫原

a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr??? phosphorylation site of human EGFR

包装

10 tests in Plastic ampoule

应用

Immunoblotting (1:1000)

警告

Toxicity: Standard Handling (A)

外形

In Dulbecco′s PBS (w/o Mg2+and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.4.

重悬

Following initial thaw, aliquot and freeze (-70°C).

分析说明

Positive Control
A431 cells stimulated with EGF

其他说明

Sieg, D.J. et al. 2000.Nat. Cell. Biol.2,249.
Barbier, A.J. et al. 1999.J. Biol. Chem.274,14067.
Biscardi, J.S., et al. 1999.J. Biol. Chem.274,8335.
Hashimoto, A. et al. 1999.J. Biol. Chem.274,20139
Poppleton, H.M. et al. 1999.Arch. Biochem. Biophys.363,227.
The immunogen sequence is 100% conserved in mouse and rat and 93% conserved in chicken EGFR, but cross-reactivity has not been tested. When diluted to 1:1000 there is sufficient antibody to perform 10 miniblots (10 T) at 10 ml per blot. Variables associated with assay conditions will dictate the proper working dilution.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

生物来源 rabbit
质量水平 100
antibody product type primary antibodies
克隆 polyclonal
形式 liquid
包含 ≤0.1% sodium azide as preservative
species reactivity human
manufacturer/tradename Calbiochem?
储存条件 OK to freeze
avoid repeated freeze/thaw cycles
同位素/亚型 IgG
运输 wet ice
储存温度 ?70℃

安全信息

储存分类代码 10 - Combustible liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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