PhosphoDetect Anti-PKR (pThr⁴⁵¹) Rabbit pAb

半岛bd体育手机客户端 说明

一般描述

Recognizes the ~65-68 kDa PKR protein phosphorylated at Thr⁴⁵¹.
Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
This PhosphoDetect Anti-PKR (pThr???) Rabbit pAb is validated for use in Immunoblotting, Immunohistochemistry for the detection of PKR (pThr???).
Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phophorylation site, followed by immunoaffinity chromatography. Recognizes the ~65-68 kDa PKR protein phosphorylated at Thr⁴⁵¹.

免疫原

a synthetic phosphopeptide corresponding to amino acids surrounding the Thr??? phosphorylation site of human PKR (double-stranded RNA-dependent protein kinase)

包装

10 tests in Plastic ampoule

应用

Immunoblotting (1:1000)

Immunohistochemistry (see comments)

警告

Toxicity: Standard Handling (A)

外形

In Dulbecco′s PBS (without Mg⁺²or Ca⁺²), 0.1% BSA, 50% glycerol, pH 7.3.

重悬

Following initial thaw, aliquot and freeze (-70°C).

分析说明

Positive Control
IFNγ-stimulated HeLa cells.

其他说明

When used at a 1:1000 dilution there is sufficient antibody for 10 blots at 10 ml/blot. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the optimal dilution.
Cuddihy, A.R., et al.1999.Oncogene8,2690.
Srivastava, S.P., et al. 1998.J. Biol. Chem.273,2416.
Clemens, M.J. 1997.Int. J. Biochem. Cell Biol.29,945.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
antibody product type	primary antibodies
克隆	polyclonal
形式	liquid
包含	≤0.1% sodium azide as preservative
species reactivity	mouse, human
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze avoid repeated freeze/thaw cycles
同位素/亚型	IgG
运输	wet ice
储存温度	?70℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable