半岛bd体育手机客户端 说明

应用

5-Chloro-2′-deoxyuridine has been used to:

• study cell cycle dynamics by immunohistochemical analysis and double S-phase labeling using animal models
• analyze cell cycle length using an animal model
• label HEK293T cells for molecular combing assay

包装

100 mg in poly bottle

生化/生理作用

DNA labeled with 5-chloro-2′-deoxyuridine (CldU) serves as an effective tool to analyze and quantify DNA replication, repair, and recombination. CldU is a potent mutagen, clastogen, and toxicant. It is used as a thymidine analog and is found to alter the dNTP pools and might lead to cell-cycle arrest. CldU produces sister-chromatid exchange but has less response to ionizing radiation compared to other thymine analogs. 5-Chloro-2′-deoxyuridine (CldU) is used to study the miscoding potential of hypochlorous acid damage to DNA and DNA precursors. When used with antibody based immunofluorescent imaging, 5-Chloro-2′-deoxyuridine incorporation may be used in protocols to identify sites of DNA replication. CldU may be used as a labeling substrate in conjunction with other halogenated uridine labeling substrates such as iododeoxyuridine (IdU).

基本信息

经验(实验)分子式	C9H11ClN2O5
分子量	262.65
MDL编号	MFCD00006531
PubChem化学物质编号	24892910
NACRES	NA.51

半岛bd体育手机客户端 性质

质量水平	100
测定	≥98% (HPLC)
形式	powder
溶解性	1 M NH4OH: 20 mg/mL, clear, colorless to faintly yellow
储存温度	?20℃
SMILES string	OC[C@H]1O[C@H](C[C@@H]1O)N2C=C(Cl)C(=O)NC2=O
InChI	1S/C9H11ClN2O5/c10-4-2-12(9(16)11-8(4)15)7-1-5(14)6(3-13)17-7/h2,5-7,13-14H,1,3H2,(H,11,15,16)/t5-,6+,7+/m0/s1
InChI key	NJCXGFKPQSFZIB-RRKCRQDMSA-N

安全信息

象形图
警示用语：	Warning
危险声明	H302 + H312 + H332 - H351
预防措施声明	P202 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P308 + P313
危险分类	Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 2
储存分类代码	11 - Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	dust mask type N95 (US), Eyeshields, Gloves